摘要
目的:研究翠绿针毛蕨Macrothelypteris viridifrons的化学成分及其抑制肿瘤细胞增殖作用。方法:利用硅胶、C18反相硅胶、Sephadex LH-20凝胶等色谱技术对其提取物进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定,以MTT法测试各化合物对MOLT-4,Hep G2,A-549,MCF-7,HT-29,PC-3肿瘤细胞增殖的抑制作用。结果:分离得到5个具有特殊B环结构的黄酮类化合物,分别鉴定为:原芹菜素(1),4'-羟基-原芹菜素(2),4'-羟基-原芹菜素4'-O-β-D-葡萄糖苷(3),5,7-二羟基-2-(1,2-异丙二氧基-4-酮-环己-5-烯)-色原酮(4),5,7-二羟基-2-(1-羟基-2,6-二甲氧基-环己-4-酮)-色原酮(5)。结论:所有化合物均首次从翠绿针毛蕨中分离得到,化合物1,4和5对6种人肿瘤细胞增殖有明显的抑制作用,且呈现出良好的剂量依赖性。
Objective：To study the chemical constituents of Macrothelypteris viridifrons and their anti-proliferative effects on tumor cell.Method：The compounds were isolated by column chromatography with silica gel,C18 reverse-phase silica gel,sephadex LH-20,and their structures were elucidated on the basis of physiochemical propertities and spectral analysis.The antitumor activities of all compounds were tested with MOLT-4,Hep G2,A-549,MCF-7,HT-29,PC-3 tumor cell lines.Result：Five compounds were isolated and identified as protoapigenone（1）,protoapigenin（2）,protoapigenin-4′-O-β-D-glucopyanoside（3）,5,7-dihydroxy-2-（1,2-isopropyldioxy-4-oxo-cyclohex-5-enyl）-chromen-4-one（4）,5,7-dihydroxy-2-（1-hydroxy-2,6-dimethoxy-cyclohex-4-oxo）-chromen-4-one（5）,respectively.Conclusion：All compounds were obtained from this plant for the first time.Compounds 1,4 and 5 showed strong anti-proliferative effects on six tumor cells,which were in concentration-dependent manner.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第5期582-584,共3页
China Journal of Chinese Materia Medica
基金
教育部高等学校博士学科点专项科研基金(20090142110021)
关键词
翠绿针毛蕨
黄酮
抗增殖作用
Macrothelypteris viridifrons
flavonoid
anti-proliferative effect
