摘要
为了解决环境中镉污染问题,研究了实验室培养的嗜镉菌吸附镉的条件和机理,讨论了时间、镉离子的质量浓度和菌液的质量浓度对吸附效果的影响。试验结果表明:在一定试验条件下,吸附时间1.5 h,吸附趋于平衡,镉离子吸附率可达80%;嗜镉菌对镉的最低吸附量为10 mg/g,最高吸附量可达50 mg/g。红外表征表明:嗜镉菌表面多糖、蛋白质的羟基峰和羰基峰均有不同程度的紫移,且糖苷峰、羟基峰的峰宽略增,说明菌体吸附主要是表面基团的作用所致。原子力显微镜表征可见,吸附后的菌体略收缩。这进一步说明该吸附是以表面基团作用为主的过程,在一定程度上对菌细胞内部的胁迫吸附有影响。
In order to solve the problem of cadmium pollution within the environment,the adsorptive conditions and mechanism of cadmium ion with cadmium-resistant strain cultivated by laboratories are conducted.The effects of adsorption time,mass concentration of cadmium ion,mass concentration of resistant strain on adsorption are discussed.Results indicate that adsorption can reach a balance when the adsorption time is 1.5 h;cadmium ion removal can reach up to 80 %.The lowest adsorbance of the strain is 10 mg/g and the highest is 50 mg/g.IR spectra of the strain shows that the hydroxyl and carbonyl groups of polysaccharide and protein exhibit red shifts in various degrees,the peak width of hydroxyl and carbonyl groups widens a little bit,which shows that the adsorption is mainly resulted from the function of the surface groups.AFM detections indicate that the mycelium shrinks a little after the adsorption,which illustrates that the adsorption mainly occurs on the surface and can affect the stress adsorption in cells to a certain extent.
出处
《黄金》
CAS
北大核心
2011年第3期60-62,共3页
Gold
关键词
嗜镉菌
镉离子
吸附条件
机理
cadmium-resistant strain
cadmium ion
adsorption condition
mechanism
