摘要
目的对14株上海市崇明地区2005年O139群霍乱弧菌菌株进行脉冲场凝胶电泳(PFGE)及核糖体分型分析。方法用PFGE、核糖体分型对霍乱弧菌进行分析。结果 14株O139群霍乱弧菌产毒株经核糖体分型分析为同一型;而PFGE双酶切分型结果为其中10株为同一型,其余4株为高度同源性。结论上海市崇明地区2005年流行的O139群霍乱弧菌核酸指纹分型基本一致。
Objective To understand the genotypes of 14 strains of Vibrio cholerae O139 isolated in Chongming in 2005. Methods Pulsed-field gel electrophoresis （PFGE） and ribotyping were conducted to analyze the Vibrio cholerae strains. Results Ribotyping indicated that the genotypes of all the O139 strains were same; but PFGE indicated that 10 of 14 strains of Vibrio cholerae O139 belonged to same type, the other 4 strains were highly homologous. Conclusion The genotypes of Vibrio cholerae O139 circulating in Chongming in Shanghai in 2005 were similarly.
出处
《疾病监测》
CAS
2011年第2期159-160,168,共3页
Disease Surveillance
关键词
霍乱弧菌
脉冲场凝胶电泳
核糖体分型
Vibrio cholerae
pulsed-field gel electrophoresis
dbotyping
