摘要
为研究ｓｂｅ１ 基因的表达调控机理，籼稻ＩＲ３６ 品种的ｓｂｅ１ 基因被克隆。经测序后与已报告的ｓｂｅ１ 基因顺序相比，ＩＲ３６ 水稻品种ｓｂｅ１ 基因５’上游区顺序中除了有分散的３２ 个碱基差异外，值得注意的是缺少一段３３５ ｂｐ 长的ＴｏｕｒｉｓｔＯｓ６ 序列，并在缺失的位置上留下转座子切离后的特征性足印顺序，这表明ＩＲ３６ 品种的ＴｏｕｒｉｓｔＯｓ６ 已从ｓｂｅ１ 基因中切离。因而为ＴｏｕｒｉｓｔＯｓ６
To study the regulatory mechanism of the sbe1 gene, it was cloned from an indica rice cultivar IR36 (Figs.1C, 4, 5A). The nucleotide sequence of this sbe1 gene showed significant homology to the sequence of the sbe1 gene reported by Kawasaki, except the absence of a 335 bp sequence of Tourist Os6 in the 5' flanking region of sbe1 gene from indica rice cultivar IR36 (Fig. 5). Moreover, a foot print remained at the site where it was inserted (Fig.5B). These results indicate that Tourist Os6 had been excised from the sbe1 gene of cultivar IR36 during evolution or cultivation; in other words, this finding provided a powerful evidence for transposibility of Tourist Os6 in rice genome.
基金
国家自然科学基金
