摘要
本研究方法为ＩＳＯ９３０８－１规定的滤膜法测定水中大肠菌群的方法，该方法操作简便只需分离和证实两步实验即可得出结果；分离培养基上的阴阳性菌落颜色分明，易于分辩；所用培养基不含致癌物质。本研究方法的准确度高，回收率的均值为９４７％，批内变异系数小于１０％，检出限为５００ｍｌ水样可检出１个ＣＦＵ，检出率是传统方法的３１倍。本研究还探讨了分离培养基的ｐＨ值对菌落颜色的影响。本研究内容经查新在国内尚无报道。
This
method is the ISO 9308-1, the Membrane Filtration Method for Coliform Organisms in Water. It's
easy to operate. Two stepsisolation and confirmation are only needed to get the result. On the
isolation media, the color of the negative colony and that of positive one are easy to
distinguish. The media used in the method doesn't contain the carcinogen. This method has the
high accuracy. The average figure of the recovery ratio is 94.7%. CV is below 10%. The limit of
detection is one CFU out of 500ml water. The detection ratio of this method is 3.1 times as much
as the traditional method. In this method, the effect of pH of the isolation media on the colonies
color is also studied. There is no other reprot about this area in China.
出处
《中国微生态学杂志》
CAS
CSCD
1999年第4期231-232,共2页
Chinese Journal of Microecology
关键词
水
滤膜法
大肠菌群
国际标准
Water\
Membrane filtration method\ Coliform organisms\ International standard
