摘要
目的研究白三烯受体(CysLT1)中927T/C(rs320995)位点基因多态性在温州地区汉族儿童中的分布特征及其对哮喘临床特征的影响。方法采用DNA测序法检测122例汉族哮喘患儿(哮喘组)和100例汉族健康体检儿童(健康对照组)CysLT1927T/C位点的基因多态性;采用MS/Paedtric肺功能仪检测肺功能。结果 1.二组均存在927T/C位点基因多态性,哮喘组CC、CT和TT基因型频率分别为33.6%、18.9%和47.5%,健康对照组分别为19.0%、17.0%、63.0%,二组基因型频率分布比较差异有统计学意义(2χ=6.769,P=0.034);哮喘组男童基因型与健康对照组比较差异有统计学意义(2χ=17.776,P=0.000),哮喘组女童基因型与健康对照组比较差异亦有统计学意义(2χ=9.676,P=0.008)。2.哮喘组C、T等位基因分别为43.0%和57.0%,健康对照组分别为28.0%和72.0%,二组比较差异有统计学意义(2χ=10.747,P=0.001)。哮喘组男童等位基因与健康对照组比较差异有统计学意义(2χ=16.396,P=0.000),哮喘组女童等位基因与健康对照组比较,差异无统计学意义(2χ=0.015,P=0.902)。3.哮喘组中,927T/C位点3种基因型特应性体质、家族特应性及哮喘严重程度比较,差异均无统计学意义。结论 1.温州地区汉族儿童CysLT1基因927T/C位点基因多态性与温州地区汉族人群哮喘易感性相关,且在性别上无明显差异。2.该位点C等位基因的男童携带者患哮喘的危险性为T等位基因携带者的2.755倍,但哮喘患病率与C等位基因的女性携带者无明显相关。3.温州地区汉族儿童中CysLT1基因927T/C位点基因多态性与特应性体质、家族特应性及哮喘严重程度无明显相关。
Objective To investigate the single nucleotide polymorphisms（SNPs） of cysteinyl-leukotriene（CysLT1） 927T/C（rs320995） in Chinese Han children in Wenzhou area and explore the correlations between gene polymorphisms of the SNPs and the susceptibility of asthma,atopic disease,atopy of family and asthma severity.Methods Sequence analysis was used to detect the SNPs of 122 Chinese Han children with asthma（asthma group） and 100 healthy children（healthy control group）.Lung function levels were measured using the lung function measuring equipment of MS/Paedtric.Results 1.CysLT1 927 T/C gene polymorphisms were present in both the asthma group and the healthy control group,with a CC frequency of 33.6% vs 19.0%,CT frequency of 18.9% vs 17.0% and TT frequency of 47.5% vs 63.0%,and there were significant differences in the genotype frequency between the 2 groups（χ^2= 6.769,P=0.034）.The frequency of genotype of male in asthma group compared with that of the male in healthy control group,there were significant differences between the 2 groups（χ2=17.776,P=0.000）.The frequency of genotype of female in asthma group compared with the genotype of female in healthy control group,there were also significant differences between the 2 groups（χ2= 9.676,P=0.008）.2.The frequency of allele was 43.0%（C） and 57.0%（T） in the asthma group,28.0%（C） and 72.0%（T） in healthy control group,respectively,and there were significant diffe-rences between the 2 groups（χ2=10.747,P=0.001）.The frequency of allele of male in asthma group compared with the allele of male in healthy control group,there were significant differences between the 2 groups（χ^2=16.396,P=0.000）.The frequency of allele of the female in asthma group compared with the allele of female in healthy control group,there were no significant differences between the 2 groups（χ^2= 0.015,P=0.902）.3.The findings showed no significant differences were found in atopic disease and atopy of family and asthma severity among the 3 genotype
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第4期254-256,303,共4页
Journal of Applied Clinical Pediatrics
基金
浙江省人口和计划生育科技项目
浙江省医学会临床科研基金(2008ZYC26)
关键词
白三烯受体
基因
单核苷酸多态性
哮喘
儿童
cysteiny-lleukotriene
gene
single nucleotide polymorphism
asthma
child
