摘要
目的以獐牙菜苦苷为底物,用黑曲霉菌对獐牙菜苦苷进行生物转化,并对转化条件进行优化。方法以獐牙菜新素生成率和獐牙菜苦苷转化率为指标,考察不同培养时间、不同碳源、不同氮源、不同种类金属离子、磷酸盐、生长因子(酵母膏)、接种量、底物加入量、不同初始pH值、不同温度条件,优化獐牙菜苦苷在黑曲霉培养液中的转化条件。结果优化后的培养条件为:葡萄糖10 g/L,蛋白胨5 g/L,酵母膏5 g/L,KH2PO4 5 g/L,MgSO4 1 g/L,CaCl2 1 g/L,初始pH 6.0,接种量0.5%,底物加入量1 mg/mL,28℃,培养5 d。结论黑曲霉转化獐牙菜苦苷生成獐牙菜新素的生成率稳定在8%左右。
Objective With swertiamarin as the substrate,biotransformation conditions of swertiamarin were optimized by Aspergillus niger.Methods With productivity ratio of EHPO {（Z）-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano [3,4-c] pyridine-1-one} and transformation ratio of swertiamarin as indexes,training time,carbon resource,nitrogen resource,metal ions,phosphate,growth factor（yeast extract paste）,inoculum size,substrate concentration,initial pH value,and tempuraure were evaluated to optimize the biotransformation of swertiamarin.Results It confirmed the optimization cultivating condition that glucose concentration was 10 g/L,peptone concentration was 5 g/L,yeast extract paste was 5 g/L,KH2PO4 concentration was 5 g/L,MgSO4 and CaCl2 concentration was 1 g/L,initial pH value was 6.0,inoculum size was 0.5%,substrate concentration was 1 mg/mL,cultivation temperature was 28 ℃,and cultivating time was 5 d.Conclusion The biotransformation ratio for EHPO into swertiamarin biotransformed by A.niger could steadily be about 8%.
出处
《中草药》
CAS
CSCD
北大核心
2011年第2期257-261,共5页
Chinese Traditional and Herbal Drugs
基金
天津市自然科学基金(08JCZDJC24700
10JCZDJC21400)
关键词
獐牙菜苦苷
獐牙菜新素
黑曲霉
培养条件
生物转化
swertiamarin
EHPO {（Z）-5-ethylidene-8-hydroxy-3
4
5
6
7
8-hexahydro-1H-pyrano [3
4-c] pyridine-1-one}
Aspergillus niger v.Tiegh.
cultivating condition
biotransformation
