摘要
采用甲状腺细胞培养方法,研究α-干扰素(IFN-α)、γ-干扰素(IFN-γ)和肿瘤坏死因子(TNF-α)对甲状腺细胞生成甲状腺球蛋白(Tg)的调节作用。结果显示:在10^(-3)～10~3u/ml的浓度范围内,IFN-α、IFN-γ及TNF-α均可显著抑制甲状腺细胞基础状态下Tg的分泌;IFN-α不影响TSH的刺激效应,但IFN-γ和TNF-α对TSH诱导的Tg释放具有双重调节作用,低浓度时(10^(-3)～10^(-1)u/ml),两种细胞因子均促进Tg的分泌,而高浓度(10~3u/ml)则呈现出明显的抑制作用。提示细胞因子是一种重要的甲状腺生物调节剂,其含量和/或甲状腺对其反应性的异常是甲状腺功能变化的重要原因。
The effect of interferon-α(IFN-α) , interferon-γ(IFN-γ) and tumor necrosis factor-α on thyroglubulin(Tg) production in cultures of human thyroid cells obtained from patients with Graves' disease were studied. The results showed that these cytokines at the concentrations of 10-3-103 u/ml significantly inhibited basal Tg secretion. IFN-α showed no influence on TSH-induced Tg generation. However, TNF-α and IFN-γ had dual effect on TSH-stimulated Tg release. At lower concentrations(10-3-10-1 u/ml), they increased Tg production, while at 103 u/ml exerted an inhibitory effect. The results indicated that these cytokines might act as circulating or/and local moduktor of thyroid gland for Tg generation and might play an important role in the pathogenesis of thyroid diseases.
出处
《放射免疫学杂志》
CAS
1999年第4期203-207,共5页
Journal of Radioimmanology
基金
江苏省自然科学基金
关键词
肿瘤坏死因子
干扰素
甲状腺球蛋白
Thyroid Cytokine Interferon Tumor necrosis factor Thyroglobulin
