摘要
目的了解2009年临床分离菌的分布和耐药特征,指导临床合理使用抗菌药物。方法采用纸片扩散法进行抗菌药物敏感性试验并对肠杆菌科细菌进行超广谱β-内酰胺酶(ESBLs)检测,按美国临床实验室标准化协会2006年标准判定药敏结果,用WHONET 5.4软件进行数据分析。结果 2009年该院共分离细菌134株,其中革兰阴性杆菌106株,占79.1%,革兰阳性球菌28株,占20.9%。大肠埃希菌和溶血葡萄球菌分别占革兰阴性杆菌和革兰阳性球菌的首位。大肠埃希菌和肺炎克雷伯菌中ESBLs的检出率分别为37.5%和21.4%。葡萄球菌属中耐甲氧西林金黄色葡萄球菌和凝固酶阴性葡萄球菌的检出率分别为50.0%和91.3%。从肠球菌属中检出1株粪肠球菌,为耐高浓度氨基糖苷类。结论从该院患者分离的细菌耐药性比较严重,应该加强监测,指导临床合理使用抗菌药物。
Objective To investigate the distribution and antibiotic resistance status of clinical isolates in our hospital during 2009 to guide rational drug use in clinic.Methods Kirby-Bauer method was used to conduct the susceptibility testing and ESBLs detection.The results were analyzed by WHONET 5.4 software according to CLSI 2006.Results Of the 134 clinical isolates,79.1%（106/134）were Gram-negative bacilli and 20.9%（28/134）were Gram-positive cocci.E.coli was the most frequently isolated pathogen in Gram-negative bacilli.Staphylococcus haemolyticus was the most common pathogen in Gram-positive cocci.ESBLs were produced in 37.5% of E.coli and 21.4% of K.pneumoniae isolates respectively.About 50% of S.aureus isolates were MRSA,91.3% of coagulase negative Staphylococcus isolates were methicillin-resistant.A strain of high level aminoglycoside resistance E.faecalis was identified.Conclusion Antimicrobial resistance in clinical bacterial isolates is a very serious problem in our hospital.Antimicrobial agents should be used rationally based on the results of susceptibility testing.
出处
《检验医学与临床》
CAS
2011年第5期569-571,共3页
Laboratory Medicine and Clinic
关键词
抗药性
细菌
革兰阳性球菌
革兰阴性杆菌
微生物敏感性试验
drug resistance
bacterial
Gram positive coccus
Gram negative bacillus
microbial sensitivity test
