摘要
目的:分离、培养小鼠味蕾细胞。方法:以小白鼠为研究对象,取其舌部组织,制成石蜡切片,从舌上皮分离出味蕾细胞置于Ⅰ型鼠尾胶原包被的含复合培养基的培养板上进行原代培养。结果:显微镜下培养的味蕾细胞与石蜡切片中味蕾细胞形态相似。结论:通过酶消化法可以进行小鼠味蕾细胞原代培养,并为后续味蕾细胞及苦味物质研究奠定技术基础。
Objective：To isolate and culture mouse taste bud cells.Methods： The tongue tissues were obtained from the mice by preparing the paraffin serial sections to isolate the taste bud cells from the lingual epithelia,and the cells were cultured in the plates coated with rat tail tendon collagen type I complex medium for primary culture.Results： Microscopically,the taste bud cells resembled the cells from paraffin sections.Conclusion： The taste bud cells of mouse may be primarily cultured by enzyme digestion,which potentially lays the foundation for further study of the cells and taste perception of bitter element.
出处
《皖南医学院学报》
CAS
2011年第1期1-3,共3页
Journal of Wannan Medical College
基金
中国烟草总公司科研项目
关键词
味蕾细胞
形态观察
原代培养
石蜡切片
taste bud cells
morphology observation
primary culture
paraffin sections
