摘要
目的研究青风藤中生物碱类成分。方法青风藤干燥藤茎用95%乙醇溶液加热回流提取,减压回收乙醇,所得浸膏利用硅胶柱色谱、反相Rp-18柱色谱、Sephadex LH-20柱色谱及重结晶等方法进行分离、纯化,通过1H-NMR、13C-NMR、EI-MS及理化常数对分离化合物进行结构鉴定。结果从青风藤提取物中分离得到6个生物碱类成分,分别鉴定为巴马亭(Ⅰ)、表小檗碱(Ⅱ)、8-demethoxyrunanine(Ⅲ)、碎叶紫堇碱(IV)、telitoxine(V)、去氢碎叶紫堇碱(Ⅵ)。结论化合物Ⅰ、Ⅱ、ⅢⅤ、Ⅵ为首次从青风藤中分离得到。
Objective To study the alkaloids of Sinomenium acutum.Methods The dried stems of Sinomenium acutum were extracted with 95% ethanol and the solvent was removed by rotary evaporation to give an extract.Column chromatograghy on Silica gel,Rp-18,Sephadex LH-20 and recrystallization were applied for the isolation and purification of the constituents.The structures were elucidated by their physicochemical properties and spectral data.Results Six alkaloids were obtained and identified as palmatine（Ⅰ）,epiberberine（Ⅱ）,8-demethoxyrunanine（Ⅲ）,（±）-cheilanthifoline（Ⅳ）,telitoxine（Ⅴ）,dehydrocheilanthifoline（Ⅵ）.Conclusion Compounds Ⅰ,Ⅱ,Ⅴ,Ⅵ were isolated from sinomenium acutum for the first time.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第2期327-328,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家"973"前期重大基础研究前期研究专项(No.2007CB516813)
国家重点基础研究发展"973"计划课题项目(No.2006CB708518)
贵州省中药现代化项目(No.(2006)5032)
中国科学院"西部之光"计划资助项目
关键词
青风藤
生物碱
化学成分
Sinomenium acutum
Alkaloids
Chemical constituent
