摘要
目的：研究糖皮质激素（ＧＣＯＲ）诱导小鼠记忆功能降低及其作用机理。方法：采用Ｆｕｒａ２／ＡＭ 钙离子荧光指示剂和双波长荧光分光光度计测定细胞内［Ｃａ２＋ ］ｉ 浓度；用透射电镜和流式细胞仪分别检测细胞凋亡（ＰＣＤ） 。结果：ＧＣＯＲ 可分别诱导成年及老龄小鼠机体免疫功能降低，胸腺及脾脏脏器指数减小，老龄动物记忆功能降低，胸腺、海马细胞内Ｃａ２＋ 超载，海马神细胞ＰＣＤ细胞数增加。体外试验结果亦表明，ＧＣＯＲ 可直接诱导Ｔ 淋巴细胞和海马神经细胞［Ｃａ２ ＋］ｉ 超载，细胞凋亡。结论：糖皮质激素可诱导小鼠记忆功能降低。细胞内［Ｃａ２＋ ］ｉ 超载可能是引起小鼠免疫功能降低、智力水平下降、衰老和细胞凋亡的主要原因之一。
AIM: To study the effect of glucocorticoids(GCOR) on intelligence in mice and its mechanism. METHODS: Immunosuppresion and decrease in intelligence of adult and old mice were induced by GCOR(sc, 4 mg·kg -1 , qd×28). Calcium fluoresence indicator Fura 2/AM was used to measure free intracellular calcium ([Ca 2+ ]i). Programmed cell death(PCD) and apoptosis were measured by means of electron microscopy and flow cytometry. RESULTS: GCOR was shown to be immunosuppress and decrese intelligence as well as caused PCD of hippocampus in mature and old mice In vivo and in vitro . This increase of GCOR continued in the free intracellular calcium concentration ([Ca 2+ ]i) of thymocytes and hippocampus and caused PCD of thymocytes. CONCLUSION: GCOR was shown to be immunosuppress and decrease in intelligence and PCD in thymocytes and hippocampus resulted in sustaining increase in [Ca 2+ ]i which may be one of the main reasons for the decrease of immunity and intelligance of mice and the process of ageing and apoptosis.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第11期806-809,共4页
Acta Pharmaceutica Sinica
基金
安徽省教委资助
关键词
糖皮质激素
细胞内钙离子
记忆功能
药理
immunosuppresion
intelligence
Fura 2
intracellular free Ca 2+
apoptosis
