摘要
目的:探讨在台州地区人群中脂联素基因多态性与动脉粥样硬化(AS)之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对198例AS患者和68例对照者的脂联素基因多态性位点(-11377C/G和+276G/T)进行基因分型。结果:AS组脂联素基因SNP+276TT基因型和T等位基因频率均低于对照组;AS组与对照组SNP-11377位点各基因型之间分布无差别,但AS组SNP-11377位点C等位基因频率低于对照组。与TT基因型的临床资料相比,GT+GG基因型在C反应蛋白(CRP)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-c)以及血糖(G lu)差别有统计学意义(P<0.05)。结论:在台州地区人群中,脂联素基因SNP+276G/T与AS的发生相关,与TT型相比,携带GG+GT型者患AS的危险因素增加,G等位基因是AS的危险因素。
Objective：To investigate the relationship between adiponectin gene single nucleotide polymorphisms（SNPs） and atherosclerosis in the Taizhou population.Methods： By using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） analysis,198 AS patients and 68 controls were characterized for adiponectin gene polymorphisms （-11377C/G,and ＋276G/T）.Results： The frequency of adiponectin gene SNP＋276TT genotype and T allele were significantly lower in patients of AS than controls.There was no significant difference in adiponectin-11377C/G SNP genotype among each group,The frequency of SNP-11377 C allele were significantly lower in patients of AS than controls.There was significant difference in C reactive protein,triglyeride（TG）,total cholesterol（TC）,low-density lipoprotein（LDL-c） and glucose（Glu） between adiponectin SNP＋276TT genotype and SNP＋276GT＋GG.Conclusion： SNP276 polymorphism of adiponectin gene is associated with AS in the population of Han ethnicity in Taizhou.The risk of AS increases in GG or GT genotype compared to TT genotype.The G allele may be a risk factor of AS in Han ethnicity of China.
出处
《中国卫生检验杂志》
CAS
2011年第2期377-379,共3页
Chinese Journal of Health Laboratory Technology
关键词
脂联素
基因多态性
动脉粥样硬化
Adiponectin
Single nucleotide polymorphism
Atherosclerosis
