摘要
目的:建立高分泌基质金属蛋白酶9(matrix metalloproteinase 9,MMP9)的皮肤成纤维细胞模型,为探讨MMP9在糖尿病足伤口愈合中的作用提供细胞模型。方法:用不同浓度的糖和同型半胱氨酸培养皮肤成纤维细胞,检测各组细胞MMP9 mRNA及蛋白表达水平和活性,筛选出MMP9表达量最高的组。结果:(1)高糖+高同型半胱氨酸组MMP9mRNA表达量(7.05±1.02)显著高于正糖组和高糖组(P均<0.01);(2)高糖+高同型半胱氨酸组MMP9蛋白表达量(206.9±33.6)pg/ml明显高于正糖组和高糖组(P均<0.01);(3)高糖+高同型半胱氨酸组MMP9蛋白活性(1.47±0.13)高于正糖组和高糖组(P均<0.01)。结论:高糖+高同型半胱氨酸联合培养成功建立体外高分泌MMP9皮肤成纤维细胞模型。
Objective：To establish a cell model of skin fibroblast with high expression of matrix metalloproteinase 9（MMP9） for studying its roles in wound healing of diabetes foots.Methods： Different concentrations of sugar and homocysteine were uesd to cultured skin fibroblasts.The expression levels of MMP9 mRNA and protein,and the activity of MMP9 protein were detected in each group.Results：（1） The expression level of MMP9 mRNA in high glucose ＋ high homocysteine group（7.05±1.02） was significantly higher than those in the normal glucose group and high glucose group（respectively,P0.01）.（2） The expression level of MMP9 protein in high glucose ＋ high homocysteine group（206.9±33.6） pg/ml was significantly higher than those in the normal glucose group and high glucose group（respectively,P0.01）.（3） The activity of MMP9 protein in high glucose ＋ high homocysteine group（1.47±0.13） was significantly higher than those in the normal glucose group and high glucose group（respectively,P0.01）.Conclusion： The cell model of skin fibroblast with high expression of MMP9 was successfully established in vitro by high glucose ＋ high homocysteine co-culture.
出处
《中国卫生检验杂志》
CAS
2011年第1期82-84,共3页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金(81070660)
广东省科技计划项目基金(2008A030201012)
广东省科技计划项目基金:(2009B091300128)
