摘要
目的:建立高效液相色谱法同时测定人参皂苷Rb_1、Rc、Rd、Rg_3、CK和Rh_2的方法。方法:采用ODSC_(18)(4.6mm×150mm)色谱柱,流动相乙腈0.05%磷酸水,梯度洗脱,流速1mL/min,检测波长203nm,柱温35℃。结果:人参皂苷Rb_1、Rc、Rd、Rg_3、CK和Rh_2分离效果良好,线性关系良好,相对标准偏差和回收率符合2010年版中华人民共和国药典要求。结论:本方法简便、准确、稳定、重现性好,可用于上述人参皂苷的含量测定。
Objective：To determine the content of ginsenoside Rbl, Rc, Rd, Rg3, CK and Rh2 simultaneously by HPLC. Methods：The samples were separated on an ODS C18 （4.6 mm × 150 mm） column using aeetonitrile-0.05 % phosphoric acid solution as the mobile phase, with the gradient elution at a flow of 1.0 mL/min and 35℃ column temperature. The wavelength of the detector was set at 203 nm. Results ： A good separation effect can be reached for ginscnoside Rb1 , Re, Rd, Rg3, CK and Rh2 which showed a good linear relationship between the peak area and the concentration of standard samples. The relative standard deviation （RSD） is good to meet the standard of pharmacopoeia of PRC. Conclusion：The method is simple, accurate, with a good reproducibility. It can be applied to qualitative and quantitative analysis of ginsenoside Rb1, Re, Rd, Rg3, CK and Rh2.
出处
《激光生物学报》
CAS
CSCD
2011年第1期87-93,107,共8页
Acta Laser Biology Sinica
基金
河南省科技厅重点科技攻关项目(082102220001)
河南省教育厅自然科学研究计划项目(2009B180006)
