摘要
为了提取高质量的蒙古黄芪(Astragalus membranaceus)基因组DNA,采用高盐十二烷基硫酸钠(SDS)法、高盐溴化十六烷基三甲基溴化铵(CTAB)法和高盐低pH值法,结果表明高盐CTAB法获得的黄芪基因组DNA纯度高,能用于PCR扩增和限制性内切酶酶切,是提取黄芪基因组总DNA的最佳方法。
High-salt SDS,high-salt CTAB,and high-salt low pH value methods were used to extract high quality Astragalus membranaceus genomic DNA.The results showed that the genomic DNA with the highest quality was extracted by high-salt CTAB method,and was preferable enough to be used as PCR template and digested by restriction enzyme completely;thus high-salt CTAB was the best DNA extraction method.
出处
《湖北农业科学》
北大核心
2011年第3期595-598,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金资助项目(60761001
61072129)
关键词
高盐
蒙古黄芪
DNA提取
high-salt
Astragalus membranaceus
DNA extraction
