摘要
研究了汽爆玉米芯酶水解及酶解液发酵生产类胡萝卜素。对影响酶解各因素考察表明:在最适酶解条件下,即温度50℃、pH值4.8、底物质量浓度50 g/L、加酶量48 FPU/g、酶解时间60 h,还原糖质量浓度达34.85 g/L。利用均匀设计对发酵条件进行了优化,最优发酵条件为:装液量23.91 mL,pH值4.85,接种量9.46%(体积分数),摇床转速190 r/min,核黄素2 mg/L,在此条件下类胡萝卜素产量可达11.24 mg/L。
Fermentation of hydrolysate from steam-exploded corn corbs to produce carotenoid was investigated. Factors affecting enzymatic hydrolysis were studied. The optimum conditions were as follows： temperature 50 ℃, pH value 4.8, substrate mass concentration 50 g/L and enzyme loading 48 FPU/g. After 60 h hydrolysis, 34.85 g/L of reducing suqars were obtained. Fermentation process for carotenoids production was optimized by a uniform design experiment. The optimum fermentation parameters were as follows： volume charge 23.91 mL, initial pH value 4.85, inoculum dosage 9.46 % （volume fraction）, rotation soeed 190 r/min, lactoflavin 2 me/L. The oroduction of carotenoid could reach 11.24 mg/L.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2011年第1期81-85,共5页
Chemistry and Industry of Forest Products
基金
四川省教育厅重点科研项目(08ZA053)
四川农业大学科技创新基金项目(00731200)
关键词
玉米芯
酶解
红酵母
类胡萝卜素
corn cob
enzymatic hydrolysis
red yeasts Rhodotorula
carotenoid
