摘要
目的通过观察不同剂量凉血活血方对大鼠肺组织肿瘤坏死因子-α(TNF-α)及转化生长因子-β(TGF-β)表达的影响,评价其对放射性肺损伤的防治效果。方法 72只Wistar种雌性大鼠随机分为模型组(A组)、凉血活血方小剂量组(B组照,射9(g3/0k gG.yd)/1、0凉次血/5活周血),方分中别剂于量第组5(、1C2组、2,61周8 g末/k分g.批d)处和死凉大血鼠活,血取方受大照剂射量肺组组(织D行组免,3疫6 g组/k化g.染d)色,对,观大察鼠大右鼠肺肺进组行织剂不量同分时割相TNF-α和TGF-β的表达。结果模型组大鼠肺组织TNF-α在第5周时达到高峰,而TGF-β在第12周和第26周时表达最强;中药各剂量组在第5周时明显抑制了TNF-α表达高峰,大、中剂量组效果更为明显;中药组TGF-β在各时相的表达明显低于模型组(P<0.01);中药大、中剂量组之间对TNF-α、TGF-β表达的影响无差别(P>0.05)。结论不同剂量凉血活血方在早期可明显抑制致炎因子TNF-α的表达,而在后期对致纤维化因子TGF-β表达的抑制更明显,中药大、中剂量组的抑制效果明显好于小剂量组和模型组。
Objective To observe the effect of Liangxuehuoxue Recipe（LXHXF）,a compound traditional Chinese medicinal preparation,on the expressions of tumor necrosis factor-α（TNF-α） and transforming growth factor-β（TGF-β） in the lung tissue of rats exposed to X-ray radiation.Methods Seventy-two Wistar rats were randomized into exposure group（group A,without treatment） and small-,middle-and high-dose LXHXF groups（groups B,C,and D treated with LXHXF at the daily doses of 9,18,and 36 g/kg,respectively）.After X-ray exposure of the right lung at 3 Gy twice a week for 5 consecutive weeks,the rats were sacrificed at the end of the 5th,12th and 26th weeks,and the lung tissues were taken for immunohistochemistry of the expressions of TNF-α and TGF-β.Results In group A,the expression of TNF-α reached the peak level at 5 weeks and TGF-β expression was the highest at 12 and 26 weeks.LXHXF,especially at the middle and high doses,obviously inhibited the expression of TNF-α at 5 weeks;the treatments also resulted in significantly lowered expressions of TGF-β at all the time points of observation as compared with those in group A（P0.01）.The high-and middle-dose groups exhibited no significant difference in the expression levels of TNF-α and TGF-β in the experiment（P0.05）.Conclusion Treatment with LXHXF can effectively inhibit TNF-α expression in the lung tissue in the early stage following radiation exposure,causing even more obvious inhibitory effect on TGF-β in the later stages.A higher dose of LXHXF produces more significant inhibitory effects on TNF-α and TGF-β expressions.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第2期266-268,共3页
Journal of Southern Medical University
基金
国家自然科学基金(30371826)
军队"十一五"课题(06MA251)~~
关键词
凉血活血方
中医药防治
放射性肺损伤
肿瘤坏死因子-α
转化生长因子-Β
Liangxuehuoxue Recipe
traditional Chinese medicine
radiation-induced lung injury
tumor necrosis factor-α
transforming growth factor-β
