摘要
目的探讨亚健康状态肾阳虚证的特征性基因差异表达谱。方法选取亚健康状态肾阳虚证患者作为研究对象,确诊为肾阳虚证患者为实验组,选择健康志愿者作为正常对照组;应用基因芯片技术,研究亚健康状态肾阳虚证的基因表达谱;结合MAS软件对差异表达基因进行分析。结果亚健康状态肾阳虚证组和正常对照组间存在差异基因表达特征图谱。获得差异表达基因共75条。其中,表达水平上调(Ratio值大于2)的有22条,表达水平下调(Ratio值小于0.5)的有53条。结论基因芯片技术可以快速、高通量筛选亚健康状态肾阳虚证的相关基因;初步获得亚健康状态肾阳虚证的相关基因。
Objective To study the gene expression profiles in kidney Yang deficiency in individuals with sub-health status.Methods The subjects identified to have Kidney-yang deficiency and sub-health status were enrolled in this study with healthy subjects as the normal control.Using gene chip technique and MAS software,the gene expression profiles in kidney Yang deficiency in the subjects with sub-health status were analyzed.Results Characteristic profiles of the differentially expressed genes were found between the kidney Yang deficiency group and normal control group.Among the total of 75 differentially expressed genes,22 were up-regulated（with a ratio greater than 2） and 53 were down-regulated（with a ratio less than 0.5） in kidney Yang deficiency group.Conclusion Gene chip technique allows rapid and high-throughput screening for gene expression in kidney Yang deficiency and sub-health status,and a initial evidence of kidney Yang deficiency-related genes has been obtained.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第2期248-251,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30772691)
高等学校博士学科点专项科研基金(20079981005)
广东省科技攻关项目(2007B060401055)~~
关键词
亚健康状态
肾阳虚证
基因芯片
基因差异表达
sub-health status
kidney Yang deficiency
gene chip
differential gene expression
