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Conserved Residues in the Subunit Interface of tau Glutathione S-transferase Affect Catalytic and Structural Functions 被引量:1

Conserved Residues in the Subunit Interface of tau Glutathione S-transferase Affect Catalytic and Structural Functions
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摘要 The tau class glutathione S-transferases (GSTs) have important roles in stress tolerance and the detoxification of herbicides in crops and weeds. Structural investigations of a wheat tau GST (TaGSTU4) show two subunit interactions: a hydrogen bond between the Tyr93 and Pro65 from another subunit of the dimer, and two salt bridges between residues Glu78 and side chains of Arg95 and Arg99 in the opposite subunit. By investigating enzyme activities, kinetic parameters and structural characterizations, this study showed the following results: (i) the hydrogen bond interaction between the Tyr93 and Pro65 was not essential for dimerization, but contributed to the enzyme's catalytic activity, thermal stability and affinity towards substrates glutathione and 1-chloro-2, 4-dinitrobenzene; and (ii) two salt bridges mainly contributed to the protein structure stability and catalysis. The results of this study form a structural and functional basis for rational design of more selective and environmentally friendly herbicides. The tau class glutathione S-transferases (GSTs) have important roles in stress tolerance and the detoxification of herbicides in crops and weeds. Structural investigations of a wheat tau GST (TaGSTU4) show two subunit interactions: a hydrogen bond between the Tyr93 and Pro65 from another subunit of the dimer, and two salt bridges between residues Glu78 and side chains of Arg95 and Arg99 in the opposite subunit. By investigating enzyme activities, kinetic parameters and structural characterizations, this study showed the following results: (i) the hydrogen bond interaction between the Tyr93 and Pro65 was not essential for dimerization, but contributed to the enzyme's catalytic activity, thermal stability and affinity towards substrates glutathione and 1-chloro-2, 4-dinitrobenzene; and (ii) two salt bridges mainly contributed to the protein structure stability and catalysis. The results of this study form a structural and functional basis for rational design of more selective and environmentally friendly herbicides.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2011年第1期35-43,共9页 植物学报(英文版)
基金 supported by grants from Beijing Forestry University (BLX2007016) the National Natural Science Foundation of China (30800873) the State Key Basic Research and Develpment Plan of China (2009CB119100)
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  • 1范志金,钱传范,陈俊鹏,王玲秀,李正名.1,8-萘二甲酸酐对高浓度单嘧磺隆协迫下玉米的解毒作用[J].农药学学报,2004,6(4):55-61. 被引量:8
  • 2DEAL L M, REEVERS J T, LARKINS B A, et al. Use of an in vitro protein synthesizing system to test the mode of action of chlo- macetamides[ J]. Weed Science, 1980, 28: 334-340. 被引量:1
  • 3WILKINSON R E. Consequences of metolachlor induced inhibition of gibberellin biosynthesis in sorghum seedlings[ J]. Pesticide Bio- chemistry and Physiology, 1988, 32 : 25-37. 被引量:1
  • 4DEVLIN R M, CUNNINGHAM R P. The inhibition of gibberellic acid induction of a-amylase activity in barley endosperm by certain herbicides[J]. Weed Research, 1970, 10(4) : 316-320. 被引量:1
  • 5SCARPONI L, BUONO D , QUAGLIARINI E, et al. Festuca arundinacea grass and herbicide safeners to prevent herbicide pal- lution [ J ]. Agronomy for Sustainable Development, 2009, 29 : 313-319. 被引量:1
  • 6BRADFORD M M. A rapid and sensitive method for the quantita- tion of microgram quantities of protein utilizing the principle of pro- tein dyebinding [ J ]. Analytical Biochemistry, 1976, 72 : 248- 254. 被引量:1
  • 7HATZIOS K K. Metabolism-based herbicide resistance:regulation- by safeners[ J]. Weed Science, 2004, 52(3): 454-467. 被引量:1
  • 8TAYLOR V, CUMMINS I, EDWARDS R, et al. Protective re- sponses induced by herbicide safeners in wheat[ J]. Environmen- ta/ and Experimental Botany, 2013, 88: 93-99. 被引量:1
  • 9SCARPONI L, BUONO D, VISCHETTI C. Effect of pretilachlor and fenclorim on carbohydrate and protein formation in relation to their persistence in rice [ J ]. Pest Managenment Science, 2005, 61 : 371-376. 被引量:1
  • 10MAMDOUH M, ALLA N, HASSAN N M. Efficacy of exogenous GA3 and herbicide safeners in protection of Zea mays from metola- ehlor toxicity[J]. Pesticide Biochemistry and Physiology, 1998, 36(11) : 809-815. 被引量:1

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