摘要
目的：研究不同剂量 Ｘ 射线全身照射对小鼠脾脏细胞凋亡的影响。方法：采用透射电镜术定性及原位末端标记（ Ｔ Ｕ Ｎ Ｅ Ｌ）术半定量地观察了电离辐射对小鼠脾脏细胞凋亡影响的时程变化及量效关系。结果：透射电镜观察到２ Ｇｙ Ｘ 射线全身照射后脾脏出现较多典型的凋亡的淋巴细胞，００７５ Ｇｙ 照射后细胞超微结构无显著变化。 Ｔ Ｕ Ｎ Ｅ Ｌ 法观察到高剂量电离辐射使脾细胞凋亡增多，而低剂量电离辐射则使脾细胞凋亡减少，剂量效应关系曲线呈“ Ｊ”型。结论：不同剂量电离辐射对脾细胞凋亡的影响不同；低剂量电离辐射使脾脏细胞凋亡减少为辐射兴奋效应的一种表现。
Objective:To study the effect of whole body irradiation (WBI) with different doses of X ray on apoptosis in mouse spleen.Methods:Time course changes and dose effect relationship of apoptosis in mouse spleen induced by WBI were observed with transmission electron microscopy (TEM) qualitatively and TUNEL method semi quantitatively.Results:Many typical apoptotic lymphocytes were found by TEM in mouse spleen after WBI with 2 Gy.No marked alterations of ultrastructure were found following WBI with 0.075 Gy.It was observed by TUNEL that the apoptosis of splenocytes increased after high dose radiation and decreased following low dose radiation (LDR).The dose effect relationship of radiation induced apoptosis showed a J shaped curve.Conclusion:The effect of different doses of ionizing radiation on apoptosis in mouse spleen was distinct.And the decrease of apoptosis after LDR is considered a manifestation of radiation hormesis.
出处
《白求恩医科大学学报》
CSCD
1999年第5期564-567,共4页
Journal of Norman Bethune University of Medical Science
基金
国家教委回国人员科研启动基金
国家自然科学基金
关键词
电离辐射
细胞凋亡
透射电镜术
ionizing radiation
spleen
apoptosis
transmission elecron microscopy
TUNEL
