摘要
目的:观察山楂饮灌胃联合愈疡生新方灌肠对小鼠免疫性溃疡性结肠炎疾病活动指数、体重变化、结肠长度、结肠炎症及溃疡愈合的作用和量效关系,为临床应用提供实验及理论依据。方法:选择100只昆明雄性小鼠,随机分为5组,即正常组、模型组、山楂饮联合愈疡生新方高剂量组(高剂组)、山楂饮联合愈疡生新方低剂量组(低剂组)、柳氮磺胺吡啶(SASP)组。除正常组外,其余各组均制作免疫性溃疡性结肠炎实验模型。于造模后第2 d开始给药,高剂组小鼠给予山楂饮0.01 g.g-1体重灌胃,愈疡生新方0.008 g.g-1体重灌肠,1次/日;低剂量组小鼠给予山楂饮0.005 g.g-1,愈疡生新方0.004 g.g-1体重灌肠,1次/日;柳氮磺胺吡啶组给予1%SASP灌肠和灌胃各0.002 g.g-1体重,1次/日;正常组、模型组给予生理盐水灌肠和灌胃各0.02 mL.g-1体重,1次/日。连续给药治疗21 d后,观察各组小鼠的疾病活动指数与体重变化。处死小鼠,取全段结肠,称结肠湿重,测肠重指数,观察结肠溃疡及炎症反应情况。结果:小鼠疾病活动指数、体重变化、各组小鼠结肠长度情况、各组小鼠结肠溃疡及炎症反应情况高剂组、低剂组、柳氮磺胺吡啶组明显优于模型组,且高剂组明显高于低剂组、柳氮磺胺吡啶组。结论:山楂饮灌胃联合愈疡生新方灌肠能明显降低小鼠免疫性溃疡性结肠炎的疾病活动指数和溃疡及炎症评分,减轻结肠充血、水肿、增厚等炎性症状,显著改善小鼠免疫性溃疡性结肠炎的结肠缩短程度;具有促进小鼠溃疡愈合、改善炎症反应的作用。其疗效与药量呈正相关。
Objective：To observe the synergism of Shanzhayin gastric gavage and Yuyang Shengxin formula clysis in the treatment of immunity ulcerative colitis（UC） mices’ model therapeutic action and the relation between the effects and the dose,provide experiment basis for clinical application.Method：Selected 100 Kunming male mice and divided randomLy into five groups：normal group;model group;the Shanzhayin gastric gavage combined Yuyang Shengxin formula clysis high dose group and the low dose group;the salicylazosulfapyridine group.Except group A,the rest groups were made models,then gave them medicine after making model two days later.The mice in Group the Shanzhayin gastric gavage combined Yuyang Shengxin formula clysis high dose filled in the stomach with Shanzhayin 0.01 g.g-1 body weight and used the enema of Yuyang Shengxin formula 0.008 g.g-1 once a day.the low dose group filled in the stomach with Shanzhayin 0.005 g.g-1 body weight and used the enema of Yuyang Shengxin formula 0.004 g.g-1 once a day.The mice of the salicylazosulfapyridine group,were given intragastric administration and enema of 1% salicylazosulfapyridine with 0.000 2 g.g-1 once a day.The mice of normal group and the model group.were given intragastric administration and enema of normal saline with 0.02 mL.g-1 once a day.After 21 days of treatment,observed the changes of disease activity index（DAI） and body weight of mice in each group.Then killed the mice and took the total colon,got the colon’s wet weight and measured the colon weight index.Surveryed the conditions of colon inflamation reaction and the change of colon histopathology.Result：The DAI of mice,the changes of weight,the length conditions of eace group,the colon conditions of inflammation and ulcer reaction of eace group：made marks according to the criterion of colon inflammation and ulcer marking,the conditions of group the Shanzhayin gastric gavage combined Yuyang Shengxin formula clysis high dose,the low dose group and the salicylazosulfapyridine group were all be
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第5期193-196,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省卫生厅科研基金项目(083710)
关键词
山楂饮
愈疡生新方
溃疡性结肠炎
正相关
Shanzhayin
Yuyang Shengxin formula
immunity ulcerative colitis
positive correlation
