摘要
使用自行设计的毛细管电泳柱端安培检测系统，采用非水介质毛细管电泳法对野菊花中蒙花苷的含量进行测定。考察了缓冲溶液浓度及其pH值、非水介质的浓度、检测电位对测定的影响。在优化实验条件下，蒙花苷的质量浓度在2．0～80mg／L范围内与峰高呈良好的线性关系，线性系数为0．9996，检出限为0．20mg／L，平均加标回收率为99％。
Using self-constructed electrochemical detection system, buddleoside was detected by high performance capillary electrophoresis （HPCE）. Several parameters affecting the determination were studied, including effects of potential of working electrode, organic concentration, running buffer, acidity and working voltage. Under the optimal conditions, a linear relationship between the peak height（Y） and the mass concentration（c） was obtained in the range of 2.0 -80 mg/L with a correlation coefficient of 0. 999 6. The determination limit was 0.2 mg/L and the average recovery was 99%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第2期225-228,共4页
Journal of Instrumental Analysis
基金
绵阳师范学院校级项目资助(MA2010003)
关键词
毛细管电泳
蒙花苷
安培检测
capillary electrophoresis
buddleoside
amperometric detection
