摘要
探索新疆裸重唇鱼的染色体数目和核型,并对新疆裸重唇鱼染色体进行C带型的描述和分析,填补领域内对这一物种研究的空白,为后续的研究打下基础。采用PHA体内注射肾细胞直接制片法研究新疆裸重唇鱼的核型,采用改进的BSG法来研究其C-带。结果表明,新疆裸重唇鱼的核型公式为2n=98=28m+30sm+12st+28t,臂数NF=156。m1、sm1、sm2、t1和t2明显比同组其他染色体大,未发现小染色体及其他异型性染色体。C-带较丰富显示出3种类型的C-带,所有染色体都有着丝粒带,部分有端粒带,有些染色体两端都有端粒带,有些只有一端有端粒带,sm5、sm12和t 3还显示深浅不一的居间带。并通过与同亚科种类进行对比分析,讨论它们的亲缘关系和系统演化。
The purpose of this study is to explore the Gymnodiptychus dybowskii Kessler chromosome number and karyotype,to describe and to analyze the C-banding of the Gymnodiptychus dybowskii Kessler chromosome.To fill the blank space in the study of this species,the foundation for follow-up study was made.The karyotype of Gymnodiptychus dybowskii Kessler was examined from its renal tissue by PHA injection-air drying method.C-band was made by modified BSG method.The results showed that the karyotype formula of Gymnodiptychus dybowskii Kessler is 2n=98=28m＋30sm＋12st＋28t,NF=156.m1,sm1,sm2,t1 and t 2 were significantly larger than those of other chromosomes in the same group.Small chromosomes and sex chromosomes with X/Y heterotypes were not found.C-band was more diverse,it had three types.All chromosomes had centromere band.Some had one telomere band and some two telomere bands.sm5,sm12 and t 3 had intermediate band with different strength.Their relationship and systematic evolution was discussed by comparing with several other species in the same subfamily.
出处
《生物学杂志》
CAS
CSCD
2011年第1期34-36,共3页
Journal of Biology
基金
石河子大学科技发展计划项目(编号:ZRKX200744)
关键词
新疆裸重唇鱼
核型
C-带
裂腹鱼亚科
Gymnodiptychus dybowskii Kessler
karyotype
C-banding
schizothoracinae
