摘要
采用AFLP技术对平泉县的2个油松纯林中油松毛虫进行了遗传多样性和遗传结构的研究。5组引物组合对90个样品DNA进行PCR扩增,并进行变性聚丙烯酰胺凝胶电泳分析,调查了2个油松林群落的环境条件。结果表明,纯林中松毛虫的遗传分化低于环境复杂的林分类型。运用POPGENE软件对2个油松毛虫种群的遗传多样性进行分析,并运用SPSS软件对影响油松毛虫种群的环境因子进行主成分分析。结果表明:2个油松纯林的油松毛虫的多态位点百分率为81.51%,总基因多样度Ht为0.259 6,基因流Nm为6.280 9,不同油松纯林中影响油松毛虫遗传多样性的各种环境因素中树高得分最高(0.473 6),说明2个油松毛虫种群之间基因交流非常强烈,林木生长状况是影响油松毛虫种群遗传多样性的主要原因,油松林的立地条件也有一定影响,物种多度的影响较小。
Using amplified fragment length polymorphism（AFLP） technique,genetic diversity and genetic structure of Dendrolimus tabulaeformis populations in 2 Pinus tabulaeformis pure forests from Pingquan County were analyzed.Moreover the environmental conditions of 2 communities were also investigated.DNA extracted from 90 individuals were amplified using 5 pairs primers.Genetic diversity of two Dendrolimus tabulaeformis populations was analyzed by POPGENE software.Finally,the principle component analysis method was used to evaluate the effect of environmental conditions on the genetic diversity of Dendrolimus tabulaeformis populations.The results showed that P was 81.51%,HT was 0.259 6 and the number of migrants per generation Nm 6.280 9.Height（0.473 6） with the highest scores was the main factor in the effects of first factor,and the main factor which influenced the genetic diversity of D.tabulaeformis in different pure forests was the growth status of forests,which was also certainly related to the site condition of the forests.The species abundance of the forests was weakly related to the genetic diversity of populations.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2011年第1期82-86,共5页
Journal of Hebei Agricultural University
基金
廊坊师范学院自然科学青年基金
国家自然科学基金(30771739)
关键词
油松毛虫
AFLP
遗传多样性
环境因子
Dendrolimus tabulaeformis
AFLP
genetic diversity
environmental factors
