摘要
目的探讨散发和具家族史食管鳞癌患者癌组织相同差异基因表达谱。方法分别将散发食管鳞癌组(Ⅰ组)和具家族史食管鳞癌组(Ⅱ组)癌及癌旁组织对应等量混合,抽提RNA合成相应cDNA,以Cy5和Cy3标志为探针,在BiostarH-140s上杂交分析。结果Ⅰ组和Ⅱ组的差异基因分别为1 855个和613个。两组表达相同差异基因60个(含表达序列标签30个)。30个基因中仅4个已有报道与食管癌相关。结论基因芯片法可高通量筛选食管鳞癌组织中异常表达的基因谱。
Objective To investigate the same in gene expression profiles of both squamous esophageal cancer carcinoma（ESCC） and their adjacent almost normal tissues related to both with and without family history. Methods The cDNA retro-transcribed from mRNA from both 3 Cases（ I group）and 6 cases（ Ⅱgroup）, which consisted of equal quantity of both squamous esophageal cancer tissues and adjacent almost normal tissues related to both with and without family history, respectively, were labeled with Cy5 and Cy3 fluorescence as a probes. The mixed probes were hybridized with a piece of Biostar H-140s single dot human whole gene chip. Results There were 1 855 genes in I group and 613 genes in the 1I group respectively,which were up regulation or down regulation. Furthermore,of 60 genes whose CyS/Cy3 ratios were simultaneous express abnormal in both Ⅰ and Ⅱ group,in addition to 30 ESTs, 30 genes were in Gengank,which included 26 genes that had not reported relating to squamous esophageal cancer except 4 genes. Conclusion Microarray may play important roles in explore the same change of both ESGC and their adjacent almost normal tissues relatedto both with and without family history.
出处
《国际检验医学杂志》
CAS
2011年第2期154-155,158,共2页
International Journal of Laboratory Medicine
基金
南京医科大学科技发展基金资助(NJ01-23)
关键词
食管肿瘤
基因表达谱
寡核苷酸序列分析
家族史
esophageal neoplasms
gene expression profiling
digonacleotide sequence analysis
family history
