摘要
目的:建立菊花配方颗粒的HPLC指纹图谱分析方法。方法:采用高效液相色谱法,以Waters Xbridge C18柱(4.6 mm×250mm,5μm)为色谱柱;以乙腈-0.1%磷酸溶液为流动相梯度洗脱;检测波长351nm;流速1.0mL.min-1;柱温30℃。结果:建立了菊花配方颗粒的指纹图谱,确定了17个共有峰,各菊花配方颗粒样品指纹图谱与对照指纹图谱相似度均在0.85以上,可以用于菊花配方颗粒的定性鉴别。结论:该方法简单、准确、重复性好,为菊花配方颗粒的质量控制提供了有效可靠的方法。
Objective：To establish a method of fingerprint analysis on Chrysanthemi Flos formula granules by HPLC.Method： The chromatographic fingerprints were obtained by HPLC with Waters Xbridge C18 column（4.6 mm × 250 mm,5 μm） by gradient elution;the solvent system composed of acetonitrile-0.1% phosphric.The wavelength was set at 351 nm;the flow rate was 1.0 mL.min-1 and the column temperature was maintained at 30 ℃.Result：Seventeen shared peaks were selected as the fingerprint peaks of Chrysanthemi Flos formula granules and good similarities with correlation coefficients higher than 0.85 were found in fingerprints between the different sam-ples of Chrysanthemi Flos Formula Granules and the standard fingerprint.Conclusion：The method with good repro-ducibility is simple and accurate;it can be used as a quality control method for Chrysanthemi Flos formula granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第4期80-82,共3页
Chinese Journal of Experimental Traditional Medical Formulae
