摘要
从福建某猪场发生疑似"高热病"死亡的小猪病料中分离到1株病毒,该病毒在Marc-145细胞上增殖致细胞病变。对分离病毒的TCID50、动物回归试验、RT-PCR鉴定及对病毒株的结构蛋白基因进行了测定与分析。结果表明:分离毒株为美洲型猪繁殖与呼吸综合征病毒(命名为PRRSV-FJ07A),TCID50为10-6.5.mL-1,对35日仔猪具有致病性,结构蛋白基因与美洲型代表株VR-2332核苷酸同源性为89.2%～95.8%,氨基酸同源性为88.1%～96.2%;与国内PRRSV-HUN4株的核苷酸同源性为90.1%～99.7%,氨基酸同源性为88.3%～100.0%;与欧洲代表株LV的核苷酸同源性为63.7%～70.0%,氨基酸同源性为59.6%～78.9%。PRRSV-FJ07A序列已登陆Genbank,登录号为GI:283100314。
PRRSV-FJ07A strain was isolated and identified.The virus was tested on the Marc-145 cell and subjected to RT-PCR,TCID50,gene sequencing of structural protein and an animal challenge test.The results showed that PRRSV-FJ07A strain belonged to the family of the North American genotype of PRRSV.Its TCID50 was 10-6.5.ml-1.Compared with VR-2332 strain,the homogeneities of nucleotide and amino acid were 89.2%-95.8% and 88.1%-96.2%,respectively,90.1%-99.7% and 88.3%-100.0%,respectively,compared with HUN4 strain,and 63.7%-70.0% and 59.6%-78.9%,respectively,compared to and LV strain.After 3 to 7 days PRRSV-FJ07A innoculation,the 35-day-old piglets showed negative serum antibody of PRRSV and appeared with clinical syndromes of PRRSV infection.The gene sequence of PRRSV-FJ07A has been submitted to Genbank issued with the number of GI： 283100314.
出处
《福建农业学报》
CAS
2010年第6期667-672,共6页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2008J0260)
福建省自然科学基金项目(2009J06012)
