摘要
目的探讨血管紧张素原基因(GT)3′-端(GT)重复多态性与藏族原发性高血压(EH)易感相关性。方法导入基于群体的病例-对照相关分析。病例组360例(M/F=168/192);对照组380人(M/F=177/203)。平均年龄分别为51.4±14.5岁和47.8±12.4岁。应用荧光标记dctp参入PCR扩增和基因扫描技术检测各片段长度及基因型。结果藏族血管紧张素基因3′-端(GT)重复序列在10种主要等位基因(频率>1%),从小到大依次称为A1(162bp),A2,A……,A10(180bp)。重复次数依次为(GT)≥1.5,(GT)16……(GT)≥24。分布频率从1.7%～27.5%;A4(GT18)为常见等位基因。高血压组A7(GT21)频率较对照组明显升高(11.3%:5.9%,χ2=5.02,P=0.023,OR=2.01,95%CI:1.05～1.87)。结论 AGT基因3′-端(GT)n多态性与藏族EH易感相关,而与汉族EH无关联。
Objective To explore association of repeat polymorphism of 3′-blanking region of angiotensinogen gene（GT） with the susceptibility to essential hypertension in Tibetan population.Methods A population-based case-control study was condueted.Study population consisted of 760 subjects,including 360 EH-patients（M/F=168/192） and 380 controls（M/F=177/203）.The GT-repeat polymorphism of AGT gene was identibied by polymerase chain reaetion（PCR） with Bluorescence labelad dCTP as substrate and by demi-automatic sequence technology.Results Association of reapeat polymorphsns at 3′-flanking region of angiotensinogen gene（GT） with EH was confined through a population based case-control study.There were 10 common alleles（frequency1%） ranging from 162bp to 180bp,for example,（GT）≥15,（GT）16……（GT）≥24.Allele4（GT18,168bp） was more frequent in both the EH patients and control subjects.Allele7（GT21） had strong positive association with genetic susceptibility to EH in Tibetan.The difference of allele frequencies between the groups was significant（11.3% vs 5.9%,χ2=5.02,P=0.023,OR=2.01,95%CI=1.05~1.87）.Conclusion The results suggest that repeat polymorphism at 3′-blanking region of AGT gene（GT） was in association with EH in Tibetans,which implicates that AGT gene might be the susceptible gene or in linkage disequilibrium with the causative genes of EH.
出处
《医学研究杂志》
2011年第1期65-68,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(39760067)
美国中华医学会基金资助项目(CMB
96-657)
"973"专项基金资助项目(2006CB504103)
医学分子生物学国家重点实验室专项经费资助项目(206204)
