摘要
比较流动培养的动脉、静脉和微血管来源内皮细胞(EC)黏附性能有无差异,探索它们在相似的流动培养条件下能否获得与移植物相当的黏附强度。采用流动培养系统,通过精密蠕动泵提供剪切力,同时提供静态培养所需其他条件,建立体外EC流动培养模型,比较三种来源途径的静态培养EC与动态培养EC在相同剪切力作用下的黏附情况,并研究EC骨架成分肌动蛋白丝的分布及在剪切力作用下的变化。流动培养后EC均沿流动方向排列并延长;静态培养EC的黏附强度以动脉EC最大,微血管EC其次,静脉EC明显低于前两者(P值均<0.05);各种来源EC动态培养后黏附强度均较相应静态培养有显著提高,动脉EC与微血管EC的细胞残留率差异无统计学意义,而静脉EC明显低于前两者(P值均<0.05)。流动培养后肌动蛋白丝均聚集成束,沿剪切力方向有序排列,致密周围带逐渐消失,形成明显的张力纤维,在微血管EC更相互联系形成一个整体。在相似的流动培养条件下,三种不同来源的EC的黏附强度较静态培养大幅度提高,且微血管来源EC能够达到与动脉EC相当的黏附强度。
This study was aimed to compare the differences of adhesion properties of endothelial cells（EC） from arteries（AEC）,veins（VEC） and capillaries（MVEC） under shear stress condition,and to explore whether they can get the same adhesive ability as graft in similar shear stress conditions.With mended parallel plate flow apparatus and peristalsis pump providing fluid shear stress used,endothelial culture models were established in vitro with the same environmental factors as steady culture.To compare the adhesion among three kinds of endothelial cells under dynamic condition and static condition,the dynamic change of cytoskeletal actin filaments and the effects of different adhesive proteins coated on the adhesion of EC to the glass were studied.The cultured endothelial cells under flow conditions were extended and arranged along the direction of flow.The adhesive ability from high to low under static condition were AEC,MVEC and VEC（VEC compared with AEC or MVEC,P0.05）,sequentially.The adhesion of endothelial cells from variety sources under dynamic culture condition was significantly increased than that of the static groups.The ratio of cell retention was not significantly different between AEC and MVEC.But VEC was significantly different（P0.05） compared with AEC or MVEC.The actin filaments（F-actin） were bundled together and arranged along the direction of flow after fluid culture.Dense peripheral band（DPB） gradually disappeared and distinct stress fibers were formed,which even interconnected to form a whole in the MVEC.The adhesion of AEC,VEC and MVEC under shear stress conditions are more significantly increased than those under the static culture conditions,and the MVEC can achieve the same adhesion as AEC.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2011年第1期157-162,共6页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30700149)
教育部博士点基金(新教师)资助项目(20070610158)
关键词
内皮细胞
流动培养
肌动蛋白丝
黏附
Endothelial cell（EC）
Flow culture
Actin filaments
Adhesion
