摘要
目的观察碱性成纤维细胞生长因子（bFGF）基因修饰的骨髓基质干细胞（BMSCs）与乙交酯-丙交酯共聚物（PLGA）构成的复合体移植于大鼠脊髓半横切处对大鼠脊髓修复的疗效。方法240只B半横切脊髓损伤大鼠随机平均分成4组（每组60只）后行相关材料移植：A组移植bFGF—BMSCs＋PLGA。B组移植bFGF—BMSCs，C组移植BMSCs，D组仅用生理盐水冲洗。术后3d、2周、3周、8周、12周时每个时间点各组随机取12只大鼠先行BBB运动功能评分及SEP监测；后处死大鼠并取损伤区脊髓组织，6只用于RT-PCR检测bFGF基因mRNA的表达，6只用于免疫组化检测神经丝蛋白-200、生长相关蛋白-43、胶质纤维酸性蛋白的表达，并采用HE染色行形态学观察。结果BBB评分结果：2周之前各组BBB评分均小于2分，差异均无统计学意义（P〉0．05）；3周以后各组评分恢复加快且A组评分高于其他组，差异均有统计学意义（P〈0．05）。SEP监测结果：术前所有大鼠均可监测到SEP，术中SEP显示达到潜伏期延长10％或波幅降低50％的脊髓损伤标准，术后3周之前所有大鼠均监测不到SEP，8周之后各组只有部分大鼠能监测到SEP。RT-PCR结果、HE染色及免疫组化结果均显示A组较其他组可明显促进轴突再生。结论大鼠脊髓半横切损伤后，单纯BMSCs移植可在一定程度上促进神经再生，移植bFGF—BMSCs可改善治疗，而移植bFGF-BMSCs＋PLGA复合体可进一步提高疗效。
Objective To investigate the effect of the implant composite of poly lactide-co-glycolide （PLGA） and bone mesenchymal stem cells （BMSCs） modified by basic fibroblast growth factor （bFGF） on injured spinal cord in rats. Methods Two hundred and forty rats were made models of spinal cord injury by hemisection of T7. They were randomly divided into 4 groups. Group A were treated with implantation of bFGF-modified BMSCs plus PLGA, group B with implantation of bFGF-modified BMSCs, group C with implantation of BMSCs without genetic modification, and group D served as control. On day 3, week 2, week 3, week 8 and week 12 after operation, 12 rats were randomly sacrificed in each group for harvest of the injured spinal cord sections, after evaluation by the Basso, Beattie ＆ bresnahan （BBB） locomotor rating scale and measurements of somatosensory evoked potentials （SEP） . The sections from 6 rats were used for reverse transcription polymerase chain reaction （RT-PCR） analysis to detect the mRNA expression level of bFGF gene, and those from the other 6 were used for the immunohistochemical examination of neurofilament-200 （NF-200）, gorwth-associated protein-43 （GAP-43）, glial fibrillary acidic proetin （GFAP） expressions and histomorphological observation of HE staining. Results The BBB scores in 4 groups were all lower than 2 points on week 2, without any significant difference among the 4 groups （ P 〉 0. 05） . After week 3, the scores increased faster in each group. Group A had a significantly higher score than the other 3 groups （ P 〈 0. 05）. SEP could be monitored in all rats before operation. During operation, the latent period was prolonged by more than 10% and the SEP amplitude declined by more than 50%. However, SEP could not be monitored in each group within 3 weeks after operation and could be monitored in part of the rats after 8 weeks in each group. The results of RT-PCR, HE staining and immunohistochemical examination in group A showed significant improvements in r
出处
《中华创伤骨科杂志》
CAS
CSCD
2011年第2期165-170,共6页
Chinese Journal of Orthopaedic Trauma
基金
基金项目：福建省自然科学基金（2007J0085）
关键词
脊髓损伤
骨髓细胞
组织工程
基因治疗
Spinal cord injury
Bone marrow cells
Tissue engineering
Gene therapy
