摘要
目的：应用血管内皮生长因子（ｖａｓｃｕｌａｒ ｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈ ｆａｃｔｏｒ， ＶＥＧＦ） 基因治疗心肌缺血。方法：雄性Ｗｉｓｔａｒ 大鼠２０ 只，结扎冠状动脉前降支造成心肌梗死模型。心脏多点注射构建的ｐｃＤ２／ＶＥＧＦ真核表达质粒。应用逆转录聚合酶链反应，ＶＥＧＦ免疫组织化学染色，心肌碱性磷酸酶染色及血管计数等方法检测ＶＥＧＦ 基因在缺血心肌中的表达及生物学作用。结果：转移ＶＥＧＦ基因后在大鼠缺血心肌中有ＶＥＧＦｍＲＮＡ 高表达， ＶＥＧＦ 免疫组化染色可见ＶＥＧＦ蛋白表达水平升高。心肌碱性磷酸酶染色显示，转移ＶＥＧＦ基因能够促进缺血心肌新生血管的形成。结论：ＶＥＧＦ基因能够在大鼠缺血心肌中获得表达，并发挥其促进新血管形成的作用。为ＶＥＧＦ 基因治疗心肌缺血奠定基础。
Objective: To investigate the efficacy of vascular endothelial growth factor (VEGF) gene therapy in the myocardial ischemia. Methods: Twenty wistar rats underwent left thoracotomy followed by the ligation of left anterior descending coronary artery. Constructed pcD 2/VEGF eukaryotic expression plasmid 30 μl (10 g·L -1 ) was directly injected into the ischemic myocardium. RT PCR, immunohistochemistry, alkaline phosphatase staining were used to detect VEGF gene expression and biological effect. Results: High levels of VEGF mRNA and protein expression were detected. There was a significant increase in the number of vessels when compared with control group ( P <0.01). Conclusion: VEGF gene application can transfect the ischemic myocardium and express VEGF protein. The VEGF gene product enhances angiogenesis and increases vessel numbers.
出处
《北京医科大学学报》
CSCD
1999年第6期489-492,共4页
Journal of Peking University(Health Sciences)
基金
国家863 计划生物技术资助项目
高等学校博士点专项科研资金
关键词
内皮生长因子
心肌缺血
治疗
基因治疗
VEGF
Endothelial growth factor Myocardial ischemia/ther Gene therapy Endothelium, vascular
