摘要
用膜片钳的方法，在培养ｗｉｓｔａｒ 大鼠乳鼠心室肌细胞上分别记录Ｔ 型、Ｌ 型、Ｂ 型钙通道的单通道活动。异马钱子碱显著激动３ 种钙通道的活动，使其开放时间延长，关闭时间缩短，开放概率增加，而对通过每一种离子通道的离子流幅值无明显影响。采用电镜观察的方法。
Single channel activities of T type,L type,and B type Ca 2+ channels were recorded on single ventricular myocardiocyte of wistar rats,using cell attached patch clamp technique.We discovered that isobrucine 50μg/ml excited the activities of the three types of Ca 2+ channels significatively,increased the open state probability and lengthened the open time,without apparent influence on the amplitude of ionic current flow through the Ca 2+ channels.The antioxidative effect of isobrucine were studied by observation under electron microscope of cultured myocardiocytes.The results indicated that X XOD destroyed the myofilaments and mitochondria.Isobrucine was able to antagonize the variations induced by X XOD.
出处
《安徽中医学院学报》
CAS
1999年第6期47-49,共3页
Journal of Anhui Traditional Chinese Medical College
基金
国家自然科学基金
江苏省教委自然科学基金
关键词
马钱子
心肌细胞
培养
钙通道
心肌
超微结构
Strychnos nux vomica
Patch clamp
Myocardium
Calcium channel
Free redicals
