摘要
根据5种主要的马铃薯病毒外壳蛋白基因序列,分别合成了5对寡聚核苷酸引物,从马铃薯病叶组织中提取出病毒总RNA,进行cDNA合成和PCR扩增,得到了与预期片段长度一致的PCR特异扩增产物。建立了马铃薯病毒检测的RT-PCR体系,并将建立的体系应用于四川省的马铃薯病毒病的检测,在基因水平上为四川省的马铃薯检测提供了新的手段。
Based on the nucleotide sequence of the coat protein（CP） gene of the five main potato virus in our country,five pairs of DNA primers were designed.The total RNA was directly extracted from virus-infected potato leaves.By the reverse transcription and polymerase chain reaction（RT-PCR）,and a specific fragment with expected length was obtained.The detection of potato virus by RT-PCR was established and applied to the Sichuan potato virus detection.A new method of the Sichuan potato virus detection was established in molecular level.
出处
《西南农业学报》
CSCD
北大核心
2010年第6期2171-2173,共3页
Southwest China Journal of Agricultural Sciences
基金
"天府英才"工程马铃薯良种繁育技术项目
