摘要
[目的]诊断由猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)和猪伪狂犬病毒(PRV)引发的猪疫病,并分析病原。[方法]采集福建某猪场发病猪的组织器官,提取病毒DNA或RNA,PCR检测病毒感染。采用ELISA方法检测CSFV、PRRSV和PRV的感染情况。[结果]测序分析和ELISA结果表明,猪场存在PRRSV、CSFV、PRV3种病毒感染。[结论]该次猪病疫情主要是由CSFV和高致病性PRRSV混合感染引起。
[Objective] To diagnose swine diseases caused by CSFV,PRRSV and PRV and analyze the pathogenic characteristics.[Method]The tissues and viscera of the diseased swine were collected from a hoggery in Fujian Province.DNA and RNA were extracted for PCR amplification and sequencing.ELISA method was used to determine CSFV,PRRSV and PRV infection.[Result] The sequencing analysis and ELISA results showed that the mixed infection was caused by CSFV,PRRSV and PRV.[Conclusion] The swine epidemic situation was mainly caused by CSFV and PRRSV.
出处
《安徽农业科学》
CAS
北大核心
2010年第34期19518-19520,共3页
Journal of Anhui Agricultural Sciences
基金
福建省区域科技重大项目(2009N3013)
福建省科技厅创新平台建设项目(2008N2005)
关键词
猪瘟
猪繁殖与呼吸综合征
混合感染
Classical swine fever
Porcine reproductive and respiratory syndrome
Mixed infection
