摘要
目的:研究狼毒大戟的抗乙肝病毒活性成分。方法:应用各种色谱技术进行分离纯化,根据化合物的波谱数据进行结构鉴定,并将化合物进行抗乙肝病毒活性的筛选。结果:分离鉴定了11个化合物,分别为5,24-甘遂二烯-3β-醇(tirucalla-5,24-dien-3-ol,1),24-亚甲基-5,24-甘遂二烯-3β-醇(24-methyltirucalla-5,24-dien-3-ol,2),大戟醇(euphol,3),丁酰鲸鱼醇(butyrosper-mol,4),24-亚甲基环阿屯醇(24-methylenecycloartenol,5),环阿屯醇(cycloartenol,6),大戟内酯E(jolkinolide E,7),泽漆内酯A(helioscopinolide A,8),异莨菪亭(isoscopoletin,9),西瑞香素(dephnoretin,10),3,3′-二甲氧基逆没食子酸4′-O-β-D-木糖苷(3,3′-di-O-methylellagic acid 4′-O-β-D-xylopyranoside,11)。结论:化合物1,2,10为首次从该属中分离得到,化合物3,4,11为首次从该植物中分离得到,化合物1,8,9,11对HBsAg和HBeAg有弱的抑制作用,化合物10对HBsAg有弱的抑制作用。
Objective： To investigate the anti-HBV constituents in the roots of Euphorbia fischeriana.Method： The compounds were isolated by various chromatographic methods and identified by spectroscopic analysis.Some compounds were tested for the anti-HBV activity.Result： Eleven compounds were isolated and identified as tirucalla-5,24-dien-3-ol（1）,24-methyltirucalla-5,24-dien-3-ol（2）,euphol（3）,butyrospermol（4）,24-methylenecycloartenol（5）,cycloartenol（6）,jolkinolid E（7）,helioscopinolide A（8）,isoscopoletion（9）,dephnoretin（10）,and 3,3′-di-O-methylellagic acid 4′-O-β-D-xylopyranoside（11）.Conclusion： Compounds 1,2 and 10 were isolated from the genus Euphorbia for the first time.Compounds 3,4 and 11 were isolated from this species for the first time.Compounds 1,8,9 and 11 showed weak anti-HBsAg and anti-HBeAg activity,while compound 10 showed weak anti-HBsAg activity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第22期3002-3006,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX9301-011)
上海市科委西部计划基金项目(074058022)
关键词
狼毒大戟
萜类
香豆素类
丹宁类
抗乙肝病毒
Euphorbia fischeriana； terpenoids； coumarins； tannins； anti-HBV activity；
