摘要
以野菊花花蕾为材料,研究其提取物的抗氧化活性。在乙醇体积分数、提取时间、料液比等单因素试验的基础上,根据中心组合试验设计原理,应用响应面分析法研究各因素的显著性和交互作用的强弱,对野菊花微波提取工艺进行优化。结果表明,微波提取野菊花清除DPPH自由基物质的优化工艺条件为乙醇体积分数50%、料液比1:27(g/mL)、提取时间51s。提取物质量浓度为0.6mg/mL时,DPPH自由基实际清除率达到83.23%,与理论预测值83.10%的绝对误差为0.13%。
Chrysanthemum indicum L. was used as the raw material to extract antioxidant materials by microwave technology. On the basis of single-factor experimental results and central composite design principle, the optimal extraction processing parameters were explored by response surface experiments through evaluating the effects of ethanol concentration, extraction time, material-liquid ratio and their cross-interactions on antioxidant activity of the extract. Results indicated that the optimal extraction processing parameters were ethanol concentration of 50% （V/V）, microwave treatment time of 51 s and material-liquid ratio of 1：27 （g/mL）. Under these optimal extraction conditions, the scavenging rate of the extract from Chrysanthemum indicum L. at the concentration of 0.6 mg/mL on DPPH free radicals was up to 83.10%, which was close to the predicted value of 83.23% with an absolute error of 0.13%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期90-94,共5页
Food Science
基金
广东省科技计划项目(2005B20401002)
关键词
响应面分析法
野菊花
微波提取
DPPH自由基
response surface method （RSM）
Chrysanthemum indicum L.
microwave extraction
DPPH free radicals
