摘要
以提取葛粉后的葛根废渣为研究对象,采用微生物和代谢物酶解提制葛根素,通过高效液相色谱法分析葛根素含量,考察影响葛根素溶出的酶解时间、酶量、pH值、固液比等参数,探索微生物与底物葛渣间的生物交互作用。实验结果表明:葛根废渣5g,纤维素酶量为底物40%,葛根废渣与水的质量比为1:10,pH值为5,在30℃酶解36h后,经乙醇浸提3h,可提取葛根素33.54mg,为葛根废渣的资源化再利用提供实验基础。
Puerarin was extracted by enzymatic hydrolysis with microbe and cellulase from waste pueraria. High performance liquid chromatography was used to determine puerarin content for exploring the effects of enzymatic hydrolysis time, enzyme addition amount, pH and material-liquid ratio as well as cross-interaction on extraction rate of puerarin. Results indicated that pueraria amount of 5 g, cellulose concentration of 40% compared with pueraria weight, pueraria-water ratio of 1：10, enzymatic hydrolysis pH of 5, enzymatic hydrolysis temperature of 30 ℃, enzymatic hydrolysis time of 36 h and ethanol extraction time of 3 h. Under the optimal enzymatic hydrolysis and extraction conditions, the yield of puerarin was up to 33.54 mg. These investigations will provide a theoretical reference for the exploration and utilization of pueraria.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期46-49,共4页
Food Science
基金
重庆市科技攻关项目(cstc
2010AC1061)
重庆市教委科技项目(cqjwKJ100708)
关键词
纤维素酶
葛根素
葛根废渣
酶解
交互作用
cellulase
puerarin
pueraria waste
enzymolysis
biological interaction
