摘要
目的:研究甲型副伤寒沙门菌gyrA基因突变情况与喹诺酮药物敏感性降低的相关性。方法:通过微量稀释法测定甲型副伤寒沙门菌对萘啶酸和3种氟喹诺酮药物的敏感性,用PCR方法扩增其gyrA基因并进行序列分析,比较不同喹诺酮药物敏感性菌株的gyrA基因突变特点和差异。结果:gyrA基因的突变率和分布对不同喹诺酮药物敏感性的影响不同,特别是在gyrA基因的Ser83位点呈现明显差别。结论:甲型副伤寒沙门菌分离株gyrA基因突变与喹诺酮药物敏感性存在关联,可导致对喹诺酮耐药或敏感性下降。
Objective：To investigate the correlation the fluoroquinolones susceptibility and the mutations in gyrA in Salmonella Paratyphi A isolates.Methods： The susceptibilities of nalidixic acid and three different quinolones were determined by agar dilution method according to the CLSI guidelines.PCR was used to amplify the gyrA gene and then sequenced.Phred/phrap/consed software was used to compare the mutations of the gyrA gene among the 35 isolates.Results： Different mutations of gyrA affected the fluoroquinilones susceptibility,especially in the isolates carrying Ser83 in gyrA.Conclusion： The quinolones susceptibily may result from the the mutations of gyrA,which even may induce the resistance or the decresced susceptibility to the quinolones.
出处
《中国卫生检验杂志》
CAS
2010年第12期3258-3259,3262,共3页
Chinese Journal of Health Laboratory Technology
基金
温州市科技局资助项目(Y20070103)
