摘要
目的:观察2009年天津市流感流行趋势、流感样病例群体暴发和甲型H1N1重症病例病原学监测情况。方法:对2009年国家级流感样病例监测哨点医院采集的流感样病例(ILI)咽拭子标本,采用病毒分离鉴定和real-time PCR检测。对疑似甲型H1N1流感病毒感染的重症病例、集中暴发病例及散发病例的呼吸道标本进行real-time PCR检测。同时对2009年5月-7月的ILI散发病例进行RT-PCR和Real-time PCR法检测,比较两种方法检测结果。对2009年9月份国家哨点医院采集标本进行核酸检测和病毒分离两种方法进行。结果:2009年共采集国家级哨点医院流感样病例标本3264份,970份流感病毒阳性,总检出率为29.7%,其中甲型H1N1亚型占55.9%(542/970株),季节性H3占26.5%(257/970株),季节性H3占26.5%(257/970株),季节性H1占7.9%(77/970),B型占0.9%(9/970株)。10月甲型H1N1明显上升,11月达高峰(91.1%),15～岁年龄段阳性率最高(72.7%)。650份疑似甲型H1N1流感重症病例中,196例(30.2%)为阳性,25～岁年龄组阳性数最高(106例),男女比例相似。321份暴发疫情标本中,190份为流感病毒阳性(59.2%)。其中甲型H1N1占44.5%(143/190),季节性H3占12.8%(41/190),B型占1.9%(6/190),未检出季节性H1。4、5月以B型为主,9～12月以甲型H1N1为主。主要为5～25岁学生。对129例散发ILI病例同时进行RT-PCR和Real-time PCR法检测,结果完全一致,均为18例流感病毒阳性。对9月份采集的474份标本进行核酸检测和病毒分离,178份核酸检测阳性,其中107例分离出毒株。结论:2009年天津地区同时存在甲型H1N1、季节性H1、H3和B型流感病毒,甲型H1N1流感病毒为优势流行型。重症病例中30.2%是由甲型H1N1流感病毒引起。暴发疫情以甲型H1N1流感病毒为主。RT-PCR和Real-time PCR法均可用于检测流感病毒。ILI核酸检测阳性率高于病毒分离阳性率。
Objective：To analyze the epidemic tendency of influenza virus,especially novel influenza A（H1N1）,in Tianjin area,and survey influenza-like illness（ILI） outbreak and to surveillance suspected severe cases infected by the novel influenza A（H1N1）.Methods： Samples collected from surveillance hospitals were detected through virus isolation and real-time PCR,and samples from ILI outbreak and suspected severe cases of novel influenza A（H1N1） were tested by real-time PCR.129 samples obtained from May to July were detected both by RT-PCR and Real-time PCR.178 influenza virus nucleic acid positive throat swabs from 474 specimens collected in September were inoculated into MDCK cells to isolate influenza viruses.Results： 3264 throat swab specimens from patients with ILI were collected in 9 national surveillance hospitals in Tianjin area in 2009.Among them,970（29.7%） were influenza viruses positive,55.9%（542/970）were novel influenza A（H1N1）,26.5%（257/970）were seasonal influenza H3N2,7.9%（77/970）were seasonal influenza H1N1,and 0.9%（9/970）were B type.Novel influenza A（H1N1） increased significantly in October,which was up to peak in November（91.1%）.The most frequently detected age was 15～25years old,and its positive rate was 72.7%.196 were novel influenza A（H1N1） positive in 650 suspected severe novel influenza A（H1N1） cases,positive rate was 30.2%.Most positive cases were 25～60 years old,no difference was observed in male and female.In 321 ILI outbreak samples,190（59.2%） were influenza virus detected,and 44.5%（143/190） of them were novel influenza A（H1N1）,seasonal H3 was 12.8%（41/190）,B was 1.9%（6/190）,no seasonal H1 was detected.In April and May,the most frequent type was B,in September to December,novel influenza A（H1N1） was predominant.The influenza outbreak happened more in 5～25 years old students.The results of RT-PCR and Real-time PCR for 124 samples were consistent with each other.107 influenza strains were isolated from 178 RNA
出处
《中国卫生检验杂志》
CAS
2010年第12期3131-3133,3135,共4页
Chinese Journal of Health Laboratory Technology
