摘要
目的:通过观察60Coγ射线照射对小鼠骨髓脂肪化的影响,建立骨髓脂肪化实验动物模型。方法:将8周龄SPF级雌性Bab l/c纯系小鼠随机分为对照组和实验组,实验组小鼠初期即接受6.0 Gy60Coγ射线全身照射处理,对照组小鼠在初期不进行照射处理。在首次60Coγ射线辐射处理后不同时间点做骨髓切片、观察比较两组小鼠骨髓病理形态的改变,应用PAS-8000病理图象分析系统分别测定各组髓腔内脂肪细胞面积。为了排除生命过程中时间的影响,在实验组小鼠首次照射处理后第48天,对照组和实验组剩余小鼠均同时接受6.0 Gy60Coγ射线全身辐射,6天后做骨髓切片、并观察检测。结果:6.0 Gy60Coγ射线照射后第6天可在小鼠骨髓组织中检测到明显脂肪组织,随着时间延长,脂肪组织越来越多,第24天达最高水平,但第48天脂肪组织则显著减少;而对照组骨髓组织从第6天到第48天均脂肪组织少见,6.0 Gy60Coγ射线全身照射后第6天才检测到明显脂肪组织。结论:60Coγ射线照射可使小鼠骨髓发生脂肪化。
Aim：To investigate the effect of 60Coγ irradiation on the bone marrow adipogenesis in mice,and to establish an animal model of bone marrow adipogenesis. Methods： 8-week-old SPF grade female Babl/c mice were randomly divided into irradiation and normal control groups.The irradiation group receive received 6.0 Gy of 60Coγ ray for the whole body,while the control group did not irradiation in the early stage.After the first irradiation,bone marrow biopsy was taken at different time points,and slices of the bone marrow were made to compare between the two groups for their pathological changes.An image analysis system,PAS-8000,was employed to measure the area of the intramedullar fat cells in the slices.In order to exclude the impact of time course during the lifespan in mice,at day 48 after the first irradiation the remaining mice of the two groups were given 6.0 Gy of 60Coγ ray for whole body irradiation.At day 6 after the second irradiation,bone marrow biopsy was taken and slices were analzed pathologically.Results： Adipose tissue was observed in mouse bone marrow 6 days after irradiation with 6.0 Gy of 60Coγ ray.There after increased adipose tissue was observed in the bone marrow,and it reached the highest level at day 24,but decreased at day 48 significantly.In contrast,the bone marrow adipose tissue in the control did not increase from day 6 to day 48,although the adipose tissue was detectable 6 days after the irradiation of 6.0 Gy of 60Coγ ray.Conclusion：60Coγ radiation can cause adipogenesis in bone marrow in mice.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2010年第6期565-568,共4页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学项目(30670902)
关键词
60Coγ射线
照射
骨髓
脂肪化
60Coγ ray
irradiation
bone marrow
adipose tissue
