摘要
目的观察不同浓度屋尘螨（HDM）对HDM过敏原Derp2基因修饰的树突状细胞（DC）表面分子的影响。方法以未转染DC为对照，不同浓度HDM处理HDM主要抗原基因Derp2转染的DC（Derp2-DC）．培养72h后流式细胞仪检测Der p2-DC表面共刺激分子CD80、CD86和MHCⅡ。结果加入HDM后．无论浓度高低。与未加入HDM相比，Der p2-DC表面分子CD80、CD86和MHC II阳性表达率均元显著变化。而加入HDM后，未转染DC表面分子CD80、CD86和MHCⅡ阳性表达率与未加入HDM相比均显著升高。结论特异性过敏原刺激不能增强过敏原基因修饰DC表面共刺激分子的表达。
Objective To investigate the effects of house dust mites （HDM） with diverse concentrations on the expressions of co stimulatory molecules of HDM major antigen gene Der p2 modified dendritic cell（DC）. Methods Der p2 gene modified D（Der p2- DC） and control DC（non-transfected DC） were treated 72 h by HDM with diverse concentrations respectively. The expressions of CD80,CD86 and MHC II ,the surface co stimulatory molecules on Der p2 -DC and control DC,were detected by FACS. Results FACS analysis showed that positive expression percentages of CD80,CD86 and MHC II on Der p2 DC treated with HDM had no significant change, compared with Der p2-DC treated without HDM. After treated with HDM, positive expression percentages of CD80, CD86 and MHC II on control DC increased significantly. Conclusions Specific allergen pulsing could not increase the expressions of costimulatory molecules of allergen gene modified DC.
出处
《国际呼吸杂志》
2011年第3期173-176,共4页
International Journal of Respiration
基金
基金项目：国家自然科学基金（30470772）
关键词
树突状细胞
共刺激分子
过敏原
免疫耐受
Dendritic cell
Co stimulatory molecule
Allergen
Immune tolerance
