摘要
目的了解铜绿假单胞菌耐药现状。方法采用多重PCR方法检测铜绿假单胞菌中的常见耐药基因。结果 39株铜绿假单胞菌耐药基因TEM、AmpC、VIM阳性率分别为84.6%、76.9%、17.9%,OXA基因扩增阴性。铜绿假单胞菌对替卡西林、妥布霉素、环丙沙星、头孢他啶耐药率均大于28.2%(11/39),耐药率最高为庆大霉素59.0%,对多粘菌素E最敏感92.3%,本组菌株中有25.6%(10/39)表现为多重耐药。结论研究表明,携带TEM、AmpC、VIM是导致本组铜绿假单胞菌耐药的重要机制,β-内酰胺类抗生素耐药性及多重耐药性均较严重。
Objective To investigate the resistance situation of Pseudomonas aeruginosa in Shenzhen. Methods The common antibiotic resistance genes in Pseudomonas aeruginosa were detected by multiplex PCR and E-test strips. Results TEM was detected in 39 stains（84.6%）.Among them,VIM was found in 17.9%（7 stains） and AmpC in 76.9%（30 stains）.The susceptibility results of antibiotics showed that the resistant rates of ticarcillin,tobramycin,ciprofloxacin,and ceftazidime were all beyond 28%.The resistant rate to gentamicin was the highest（59.0%）.The more sensitive antibiotic was amikacin. Conclusions The study indicated that these Pseudomonas aeruginosa carried genes of TEM,AmpC,and VIM,which was the essential resistance mechanism of Pseudomonas aeruginosa in local area.Multidrug resistance and Beta-lactam antibiotics are severity.
出处
《实用预防医学》
CAS
2011年第1期12-14,共3页
Practical Preventive Medicine
基金
湖南省教育厅科学研究立项并资助项目(10C0317)
关键词
铜绿假单胞菌
多重PCR
耐药基因
Pseudomonas aeruginosa
Multiplex PCR
Resistance genes
