摘要
以白菜型油菜(Brassicarapa)的亚种菜心(Brassica parachinensis)的子叶和下胚轴为外植体,研究不同浓度的NAA和6-BA配比对菜心组织培养的影响。结果表明:菜心子叶和下胚轴组织培养的再生最佳培养基分别是MS+6-BA1.5 mg/L+NAA0.2 mg/L+AgNO35 mg/L和MS+6-BA2.0 mg/L+NAA0.2 mg/L+AgNO35 mg/L;不定芽生根最佳培养基是MS+NAA0.2 mg/L。同时还研究了AgNO3浓度对菜心组织培养的影响,在培养基中附加AgNO3能提高菜心芽的诱导频率。
Cotyledons and hypocotyls of Brassica parachinensis subspecies of Brassica rapa were used as explants to study the effect of different concentration ratio of NAA and 6-BA on Brassica parachinensis tissue culture.The results showed that the best regeneration medium for cotyledons and hypocotyls of Brassica parachinensis were MS＋6-BA 1.5 mg/L＋NAA 0.2 mg/L＋AgNO3 5 mg/L and MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L＋AgNO3 5 mg/L respectively.The best root medium of adventitious buds was MS＋NAA 0.2 mg/L.It was also discussed the influence of the concentration of AgNO3 on Brassica parachinensis tissue culture.The high inducement frequency of shoot regeneration was gained by addition of AgNO3 in the medium.
出处
《北方园艺》
CAS
北大核心
2011年第1期127-129,共3页
Northern Horticulture
基金
河南省科技发展计划国际合作资助项目(084300510076)
河南省科技厅基础与前沿技术资助项目(082300430320)
关键词
白菜型油菜
植株再生
组织培养
Brassica rapa
plant regeneration
tissue culture
