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HPLC法测定大麦苗提取物中β-胡萝卜素的含量 被引量:7

Determination of β-carotene in Barley Grass Extract by HPLC
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摘要 目的建立高效液相色谱法测定大麦苗提取物中β-胡萝卜素的含量测定方法。方法色谱柱为Kromasil C18(5μm,250 mm×4.6 mm);流动相:甲醇-三氯甲烷-正己烷(50:35:15);检测波长:448nm,柱温:30℃。结果β-胡萝卜素的回收率为100.4%;线性范围为0.039 52~0.790 4μg。结论结果准确,重复性好,可用于该产品的质量控制。 Objective To establish an HPLC method for the determination of 13-carotene in Barley Grass extract.Methods HPLC was used for quantitative analysis. The Kromasil C18 column(5μm,250 mm×4.6mm) was used, and mobile phase was composed of methanol- Chloroform-normal hexane ( 50:35:15 ) . Detection wavelength was set at 448 nm at 30 ℃. Results The average recovery of β-carotene was 100.4%, and the linearity was good in the range of 0.039 52-0.790 4 gg. Conclusion The result is accurate and reproducibility is good. The method can be used for the quality control of the products.
出处 《现代中药研究与实践》 CAS 2011年第1期50-51,共2页 Research and Practice on Chinese Medicines
关键词 大麦苗提取物 Β-胡萝卜素 含量测定 HPLC Barley Grass extract β-carotene determination HPLC
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