摘要
目的建立高效液相色谱法测定大麦苗提取物中β-胡萝卜素的含量测定方法。方法色谱柱为Kromasil C18(5μm,250 mm×4.6 mm);流动相:甲醇-三氯甲烷-正己烷(50:35:15);检测波长:448nm,柱温:30℃。结果β-胡萝卜素的回收率为100.4%;线性范围为0.039 52~0.790 4μg。结论结果准确,重复性好,可用于该产品的质量控制。
Objective To establish an HPLC method for the determination of 13-carotene in Barley Grass extract.Methods HPLC was used for quantitative analysis. The Kromasil C18 column(5μm,250 mm×4.6mm) was used, and mobile phase was composed of methanol- Chloroform-normal hexane ( 50:35:15 ) . Detection wavelength was set at 448 nm at 30 ℃. Results The average recovery of β-carotene was 100.4%, and the linearity was good in the range of 0.039 52-0.790 4 gg. Conclusion The result is accurate and reproducibility is good. The method can be used for the quality control of the products.
出处
《现代中药研究与实践》
CAS
2011年第1期50-51,共2页
Research and Practice on Chinese Medicines