摘要
目的探讨5岁以下喘息儿童鼻咽分泌物中嗜酸性粒细胞(EOS)计数和嗜酸细胞阳离子蛋白(ECP)水平在喘息评估中的临床意义。方法 53例5岁以下反复喘息患儿,分为有特应性体质的喘息Ⅰ组27例,非特应性体质的喘息Ⅱ组26例;健康对照组20例,分别收集鼻咽分泌物(NPS),显微镜下计数各组NPS中细胞数,用ELISA方法检测上清液中ECP水平。结果①喘息Ⅰ组鼻咽分泌物ECP水平和EOS计数均明显高于喘息Ⅱ和对照组(P均<0.05),而喘息Ⅱ组和对照组之间无统计学差异(P>0.05);②喘息Ⅱ组鼻咽分泌物中性粒细胞明显高于喘息Ⅰ组和对照组(P均<0.05);③喘息Ⅰ组NPS中ECP水平和EOS呈正相关(r值为0.89,P<0.05),与其他细胞无相关性。结论检测NPS中EOS和ECP可以作为识别5岁以下有发展为哮喘倾向的喘息儿童的指标,并给予早期临床干预和治疗。
Objective To explore the clinical significance of eosinophils（EOS）and eosinophil cationic protein（ECP）in nasopharyngeal secretions of wheezing children under 5 years old.Methods Fifty-three children under five years old who had recurrent wheezing were divided into 2 groups：wheezing group Ⅰ with atopic body（n=27）and wheezing group Ⅱ without atopic body（n=26）.Twenty healthy children were enrolled as the control group.Nasopharyngeal secretions（NPS）were collected and disposed respectively,inflammatory cells of NPS were counted under the microscope,the level of ECP of supernatant was measured by PharmaciaUniCAP system.Result ① The levels of ECPand PBEC of NPS in wheezing groupⅠ were significantly higher than those of wheezing group Ⅱand the control group,the difference was statistically significant（P〈0.05,P〈0.01）,while wheezing group Ⅱ group and the control group had no significant difference（P〈0.05）;② The accounts of neutrophil of wheezing group Ⅱwere significantly higher than those of wheezing groupⅠ and the control group in NPS;③ ECPand PBEC of NPS have positive correlation（r值为0.89,P〈0.05）.Conclusion EOS counts and the detections of the ECP in NPS can be used as index to discriminate the children under five years old who have a tendency to develop asthma,early intervention and treatment should be given to them.
出处
《临床肺科杂志》
2011年第2期182-184,共3页
Journal of Clinical Pulmonary Medicine
