摘要
目的观察匹维溴胺对腹泻型肠易激综合征(D-IBS)模型大鼠血清以及结肠组织血管活性肠肽(vasoactiveintestinal peptide,VIP)含量、结肠组织水通道蛋白3(aquaporin 3,AQP3)表达的影响,探讨其治疗D-IBS的作用机制。方法采用慢性应激与束缚相结合方法建立D-IBS大鼠模型。采用ELISA法检测VIP含量;采用Real time-PCR法和SABC免疫组化法检测AQP3的表达。结果在血清和结肠组织中VIP的含量,与对照组[(3.092±0.613)ng/ml,(3.811±0.275)ng/ml]比较,模型组[(10.460±1.666)ng/ml,(4.495±0.341)ng/ml]与匹维溴胺组[(5.639±1.163)ng/ml,(4.063±0.534)ng/ml]均升高(P<0.05),尤以模型组升高的更为明显(P<0.01);两组AQP3的表达均下调(P<0.01),尤以模型组AQP3蛋白表达下调显著。结论匹维溴胺治疗D-IBS的作用机制之一,可能与抑制结肠组织中VIP分泌和上调AQP3表达有关。
Objective To study the serum vasoactive intestinal peptide（VIP） level and the effect of pinaverium bromide（PB） on expression of aquaporin 3（AQP3） in colon tissue and diarrhea-predominant irritable bowel syndrome（D-IBS） model of rats.Methods A D-IBS rat model was established with chronic stress plus binding limbs.Level of VIP was measured by ELISA and expression of AQP3 was detected by real-time PCR and SABC,respectively.Results The VIP level in serum and colon tissue was higher in model group（10.46±1.666 and 4.495±0.341 ng/ml） and PB group（5.639±1.163 and 4.063±0.534 ng/ml） than in control group（3.092±0.613 and 3.811±0.275 ng/ml）（P0.05）,which was particularly high in model group.The expression of AQP3 in colon tissue was remarkably decreased in model group and PB group,especially in model group（P0.01）.Conclusion PB improves D-IBS by inhibiting the secretion of VIP and up-regulating the AQP3 expression in colon tissue.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第3期233-236,共4页
Journal of Third Military Medical University
基金
国家重点基础研究发展计划(973计划
2007CB512702)~~
关键词
腹泻型肠易激综合征
血管活性肠肽
水通道蛋白3
匹维溴胺
diarrhea-predominant irritable bowel syndrome
vasoactive intestinal peptide
aquaporin 3
pinaverium bromide
