摘要
目的了解淋病奈瑟菌毒力岛(PAIs)在质粒及染色体上的分布情况。方法采用碱裂解法提取63株淋病奈瑟菌的质粒及染色体DNA,PCR扩增各PAIs基因(atlA,traG,traH)。结果共检出4.2kb,7.4kb,39.5kb,42.5kb四种质粒,总检出率为88.89%(56/63);以63株淋病奈瑟菌的染色体DNA为模板进行PCR扩增,atlA,traG和traH的阳性检出率分别为74.60%(47/63),34.92%(22/63)和68.25%(43/63);以56株淋病奈瑟菌的质粒DNA为模板进行PCR扩增,atlA,traG和traH的阳性检出率分别为75.00%(42/56),62.50%(35/56)和64.29%(36/56)。方法淋病奈瑟菌的毒力岛基因不仅存在于染色体上,而且存在于质粒上,且携带率高,分布广泛。
Objective To explore the distribution of the gonococcal pathogenicity islands （PAls） on chromosome and plasmid. Methods The plasmids and chromosome of 63 Neisseria gonorrhoeae strains were separately extracted by alkaline lysis technique. PAIs genes atlA, traG and trail were amplified by PCR and located. Resuits Of the 63 Neisseria gonorrhoeae strains, the plasmids of 4.2kb, 7.4kb, 39.5kb and 42.5kb were detected. The PCR results of PAls genes in chromosome DNA showed that the detection rates of atlA, traG and trail were 74.60% （47/63）, 34.92% （22/63） ,68.25% （43/63）, respectively. By using the same method, the detection rates of atlA, traG and trail in plasmid DNA were 75.00% （42/56）, 62.50% （35/56） and 64.29% （ 36/56）, respectively. Conclusion The gonococcal pathogenicity islands generally present not only in chromosome, but also in plasmid. Its detection rate is very high.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2011年第2期104-106,共3页
The Chinese Journal of Dermatovenereology
基金
广东省自然科学基金资助(5011580)
关键词
淋病奈瑟菌
质粒
毒力岛
Neisseria gonorrhoeae
Plasmid
Pathogenicity islands
