摘要
目的探讨紧密连接蛋白1(ZO-1)mRNA及蛋白表达在胃癌侵袭转移及预后中的意义。方法 Real-time qPCR法检测52例胃腺癌细胞ZO-1 mRNA表达,组织芯片SP免疫组织化学法检测228例胃腺癌细胞ZO-1蛋白表达定位变化情况。结果 81.1%的胃癌组织出现不同程度的ZO-1胞膜表达异位至胞质迷离表达的现象。黏膜层胃腺癌细胞ZO-1迷离表达的程度在高-中分化组与低分化组、浸润深度局限于肌层组与突破肌层组、无淋巴结转移组与有淋巴结转移组、早期胃癌组与进展期胃癌组间均有显著差别(P<0.05)。侵袭前沿区胃腺癌ZO-1迷离表达程度比黏膜层胃腺癌加剧,淋巴结转移灶较侵袭前沿区迷离表达程度降低(P<0.05)。黏膜层胃腺癌细胞ZO-1迷离表达者较正常表达者术后生存率低(P<0.05)。但黏膜层胃腺癌组织与其对应的癌旁正常胃黏膜组织ZO-1 a+mRNA表达无明显差异。黏膜层胃腺癌组织中ZO-1 a+mRNA表达变化与年龄、性别、组织学分化类型、浸润深度及淋巴结转移等未见明显相关性。结论胃腺癌细胞ZO-1迷离表达参与胃腺癌的进展,但ZO-1mRNA表达水平无明显变化。提示可能不是ZO-1 mRNA表达水平的变化,而是ZO-1迷离表达参与胃癌的发生发展。
Objective To investigate the significance of zonula occludens-1 （ZO-1） mRNA and protein expression in invasion and metastasis and prognosis of human gastric carcinomas. Methods Real-time qPCR and SP immunohistochemistry on tissue microarray were used to detect 52 cases of ZO-1 mRNA and 228 cases of protein expression in gastric cancer. Results In contrast to normal epithelium, 81.1% gastric carcinoma tissues showed different degrees of aberrant expression of ZO-1 from membrane translocated to cytoplasm. The ZO-1 aberrant degree in the mucosal layer of gastric carcinoma cells in the case of well-differentiated, invasiveness depth confined to muscularis, without lymph node metastasis and early gastric cancer was lower than those in poorly differentiated, invasiveness depth breakthrough to muscularis, with lymph node metastasis and progressed gastric cancer, respectively （P 〈 0. 05）. The ZO-1 aberrant degree in the invasive front area of gastric adenocarcinoma cell was higher than that in mucosa, but in lymph node metastases lower than that in invasive front area （P 〈 0.05）. Postoperative survival rate in case with ZO-1 aberrant expression in mucosal layer of gastric carcinoma was lower than that in ZO-1 normal expression（P 〈 0. 05）. However, ZO-1 a ＋ mRNA expression between gastric adenocarcinomas in mucosal layer and normal gastric epithelial cells in their corresponding adjacent normal gastric mucosa was not significantly different. ZO-1 a ~ mRNA expression in mucosal layer of gastric adenocarcinomas was not significantly correlated with age, sex, histological type, the invasive depth and status of lymph node metastasis. Conclusion ZO-1 aberrant expression involved in the progress of gastric adenocarcinoma, but ZO-1 mRNA expression was not significantly different. These results suggested that hot the change of ZO-1 mRNA quantity but the ZO-1 aberrant expression associated withcarcinogenesis and progression in gastric carcinoma.
出处
《解剖学报》
CAS
CSCD
北大核心
2011年第1期80-85,共6页
Acta Anatomica Sinica
基金
福建医科大学学科带头人基金资助项目(JXK200724)
福建省卫生厅青年科研课题基金资助项目(2010-02-27)
关键词
胃癌
紧密连接蛋白1
侵袭
转移
组织芯片
免疫组织化学
人
Gastric carcinoma
ZO-1
Invasion
Metastasis
Tissue microarray
Immunohistochemistry
Human
