摘要
球囊霉素是由丛枝菌根真菌分泌的一种含金属离子的糖蛋白,因被非专一性提取而称为球囊霉素相关土壤蛋白(GRSP)。由于提取条件能影响GRSP的测定值,因此研究了柑橘园土样前期灭菌、离心力和土样质量等3个提取条件对GRSP测定值的影响。结果表明,土样前期灭菌和较高的离心力(15,000×g)均能显著影响GRSP的测定值;在土样质量0.25-1.0g范围内,GRSP的测定值随着土样质量的提高而升高;经过3次和6次的连续提取,总球囊霉素相关土壤蛋白的累积量分别达到理论最大值的66.8%和92.1%以上。研究结果为GRSP测定的标准化提供了依据。
Glomalin is a soil glycoprotein produced by arbuscular mycorrhizal （AM） fungi,presently named as glomalin-related soil protein （GRSP） due to its unspecific extraction. The extraction conditions of GRSP can affect its measured value,therefore,the influences of pre-sterilization of soil sample,centrifugation and soil sample mass on the measured value of GRSP were studied with citrus orchard soil. The results showed that both pre-sterilization of soil sample and centrifugation at higher centrifugal force （15,000×g） significantly affected the measured value of GRSP,indicating in the range of 0.25-1.0g the greater of soil sample mass,the higher of the measured value showed. The cumulative contents of total GRSP exceeded 66.8% and 92.1% of the theoretical maximum,respectively. These results provide a basis for the standardization of GRSP measurement,and also contribute to the comparison among the measured values of GRSP in literatures.
出处
《菌物学报》
CAS
CSCD
北大核心
2011年第1期92-99,共8页
Mycosystema
基金
国家自然科学基金(No.30870458
31070103)
广东省自然科学基金(No.10251007002000001)
关键词
丛枝菌根真菌
蛋白提取
离心力
灭菌
土样质量
arbuscular mycorrhizal fungi
extraction of protein
centrifugal force
sterilization
soil sample mass
